SRRM2, a Potential Blood Biomarker Revealing High Alternative Splicing in Parkinsons DiseaseReport as inadecuate




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Background

Parkinson-s disease PD is a progressive neurodegenerative disorder that affects about five million people worldwide. Diagnosis remains clinical, based on phenotypic patterns. The discovery of laboratory markers that will enhance diagnostic accuracy, allow pre-clinical detection and tracking of disease progression is critically needed. These biomarkers may include transcripts with different isoforms.

Methodology-Principal Findings

We performed extensive analysis on 3 PD microarray experiments available through GEO and found that the RNA splicing gene SRRM2 or SRm300, sereine-arginine repetitive matrix 2, was the only gene differentially upregulated among all the three PD experiments. SRRM2 expression was not changed in the blood of other neurological diseased patients versus the healthy controls. Using real-time PCR, we report that the shorter transcript of SRRM2 was 1.7 fold p = 0.008 upregulated in the substantia nigra of PDs vs controls while the longer transcript was 0.4 downregulated in both the substantia nigra p = 0.03 and amygdala p = 0.003. To validate our results and test for the possibility of alternative splicing in PD, we performed independent microarray scans, using Affymetrix Exon ST1 arrays, from peripheral blood of 28 individuals 17 PDs and 11 Ctrls and found a significant upregulation of the upstream 5′ exons of SRRM2 and a downregulation of the downstream exons, causing a total of 0.7 fold down regulation p = 0.04 of the long isoform. In addition, we report novel information about hundreds of genes with significant alternative splicing differential exonic expression in PD blood versus controls.

Conclusions-Significance

The consistent dysregulation of the RNA splicing factor SRRM2 in two different PD neuronal sources and in PD blood but not in blood of other neurologically diseased patients makes SRRM2 a strong candidate gene for PD and draws attention to the role of RNA splicing in the disease.



Author: Lina A. Shehadeh , Kristine Yu, Liyong Wang, Alexandra Guevara, Carlos Singer, Jeffery Vance, Spyridon Papapetropoulos

Source: http://plos.srce.hr/



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