Visualization and Identification of IL-7 Producing Cells in Reporter MiceReport as inadecuate




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Interleukin-7 IL-7 is required for lymphocyte development and homeostasis although the actual sites of IL-7 production have never been clearly identified. We produced a bacterial artificial chromosome BAC transgenic mouse expressing ECFP in the Il7 locus. The construct lacked a signal peptide and ECFP enhanced cyan fluorescent protein accumulated inside IL-7-producing stromal cells in thoracic thymus, cervical thymus and bone marrow. In thymus, an extensive reticular network of IL-7-containing processes extended from cortical and medullary epithelial cells, closely contacting thymocytes. Central memory CD8 T cells, which require IL-7 and home to bone marrow, physically associated with IL-7-producing cells as we demonstrate by intravital imaging.



Author: Renata I. Mazzucchelli, Søren Warming, Scott M. Lawrence, Masaru Ishii, Mehrnoosh Abshari, A. Valance Washington, Lionel Feigenb

Source: http://plos.srce.hr/



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