Flow-Cytometric Phosphoprotein Analysis Reveals Agonist and Temporal Differences in Responses of Murine Hematopoietic Stem-Progenitor CellsReport as inadecuate




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Hematopoietic stem cells HSCs are probably the best-studied adult tissue-restricted stem cells. Although methods for flow cytometric detection of phosphoproteins in hematopoeitic progenitors and mature cells are available, analogous protocols for HSC are lacking. We present a robust method to study intracellular signaling in immunophenotypically-defined murine HSC-progenitor cell HPC-enriched populations. Using this method, we uncover differences in the response dynamics of several phosphoproteins representative of the Ras-MAP-KinaseK, PI3K, mTOR and Jak-STAT pathways in HSC-HPCs stimulated by Scf, Thpo, as well as several other important HSC-HPC agonists.



Author: Demetrios Kalaitzidis , Benjamin G. Neel

Source: http://plos.srce.hr/



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