Virulent Synergistic Effect between Enterococcus faecalis and Escherichia coli Assayed by Using the Caenorhabditis elegans ModelReport as inadecuate




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Background

The role of enterococci in the pathogenesis of polymicrobial infections is still debated. The purpose of this study was to evaluate the effect of virulent enterococci in the presence or absence of Escherichia coli strains in the in vivo Caenorhabditis elegans model.

Principal Findings

This study demonstrated that there was a synergistic effect on virulence when an association of enterococci and E. coli LT50 = 1.6 days±0.1 according to the tested strains and death of nematodes in 4 days±0.5 was tested in comparison with enterococci alone LT50 = 4.6 days±0.1 and death in 10.4 days±0.6 or E. coli alone LT50 = 2.1±0.9 and deaths 6.6±0.6 p<0.001. In addition, there was a relation between the virulence of E. faecalis strains alone and the virulence potential of the association with E. coli strains. Finally, in the presence of avirulent E. coli strains, enterococci have no effect LT50 = 4.3±0.5 and deaths in 10.8±0.8, independently of the level of their own virulence, demonstrating that the ‘enterococci effect’ only occurred in the presence of virulent E. coli strains.

Conclusion

This study allows a better understanding of a bacterial cooperation. Moreover, it could help to optimize the antibiotic regimen during polymicrobial infections.



Author: Jean-Philippe Lavigne, Marie-Hélène Nicolas-Chanoine, Gisèle Bourg, Jérôme Moreau, Albert Sotto

Source: http://plos.srce.hr/



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