Single Bead Affinity Detection SINBAD for the Analysis of Protein-Protein InteractionsReport as inadecuate




Single Bead Affinity Detection SINBAD for the Analysis of Protein-Protein Interactions - Download this document for free, or read online. Document in PDF available to download.

We present a miniaturized pull-down method for the detection of protein-protein interactions using standard affinity chromatography reagents. Binding events between different proteins, which are color-coded with quantum dots QDs, are visualized on single affinity chromatography beads by fluorescence microscopy. The use of QDs for single molecule detection allows the simultaneous analysis of multiple protein-protein binding events and reduces the amount of time and material needed to perform a pull-down experiment.



Author: Roberta Schulte, Jessica Talamas, Christine Doucet, Martin W. Hetzer

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents