Cooperative Stimulation of Dendritic Cells by Cryptococcus neoformans Mannoproteins and CpG OligodeoxynucleotidesReport as inadecuate




Cooperative Stimulation of Dendritic Cells by Cryptococcus neoformans Mannoproteins and CpG Oligodeoxynucleotides - Download this document for free, or read online. Document in PDF available to download.

While mannosylation targets antigens to mannose receptors on dendritic cells DC, the resultant immune response is suboptimal. We hypothesized that the addition of toll-like receptor TLR ligands would enhance the DC response to mannosylated antigens. Cryptococcus neoformans mannoproteins MP synergized with CpG-containing oligodeoxynucleotides to stimulate enhanced production of proinflammatory cytokines and chemokines from murine conventional and plasmacytoid DC. Synergistic stimulation required the interaction of mannose residues on MP with the macrophage mannose receptor MR, CD206. Moreover, synergy with MP was observed with other TLR ligands, including tripalmitoylated lipopeptide Pam3CSK4, polyinosine-polycytidylic acid pI:C, and imiquimod. Finally, CpG enhanced MP-specific MHC II-restricted CD4+ T-cell responses by a mechanism dependent upon DC expression of CD206 and TLR9. These data suggest a rationale for vaccination strategies that combine mannosylated antigens with TLR ligands and imply that immune responses to naturally mannosylated antigens on pathogens may be greatly augmented if TLR and MR are cooperatively stimulated.



Author: Jennifer M. Dan, Jennifer P. Wang, Chrono K. Lee, Stuart M. Levitz

Source: http://plos.srce.hr/



DOWNLOAD PDF




Related documents