IFN-Dependent and -Independent Reduction in West Nile Virus Infectivity in Human Dermal FibroblastsReport as inadecuate




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1

Maryland Pathogen Research Institute, College Park, MD 20742, USA

2

Department of Cell Biology and Molecular Genetics University of Maryland, College Park, MD 20742, USA





*

Author to whom correspondence should be addressed.



Abstract Although dermal fibroblasts are one of the first cell types exposed to West Nile virus WNV during a blood meal by an infected mosquito, little is known about WNV replication within this cell type. Here, we demonstrate that neuroinvasive, WNV-New York WNV-NY, and nonneuroinvasive, WNV-Australia WNV-AUS60 strains are able to infect and replicate in primary human dermal fibroblasts HDFs. However, WNV-AUS60 replication and spread within HDFs was reduced compared to that of WNV-NY due to an interferon IFN-independent reduction in viral infectivity early in infection. Additionally, replication of both strains was constrained late in infection by an IFN-β-dependent reduction in particle infectivity. Overall, our data indicates that human dermal fibroblasts are capable of supporting WNV replication; however, the low infectivity of particles produced from HDFs late in infection suggests that this cell type likely plays a limited role as a viral reservoir in vivo. View Full-Text

Keywords: West Nile virus; interferon; human dermal fibroblast; antiviral response; intracellular innate immunity West Nile virus; interferon; human dermal fibroblast; antiviral response; intracellular innate immunity





Author: Lisa I. Hoover 1,2 and Brenda L. Fredericksen 1,2,*

Source: http://mdpi.com/



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