The Carboxy Terminal Region of the Human Cytomegalovirus Immediate Early 1 IE1 Protein Disrupts Type II Inteferon SignalingReport as inadecuate




The Carboxy Terminal Region of the Human Cytomegalovirus Immediate Early 1 IE1 Protein Disrupts Type II Inteferon Signaling - Download this document for free, or read online. Document in PDF available to download.

Department of Surgery, The Ohio State University, 473 West 12th Avenue, Columbus, OH 43210, USA



Current Address: Monsanto Company, 800 North Lindbergh Blvd, St. Louis, MO 63167, USA





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Abstract Interferons IFNs activate the first lines of defense against viruses, and promote innate and adaptive immune responses to viruses. We report that the immediate early 1 IE1 protein of human cytomegalovirus HCMV disrupts signaling by IFNγ. The carboxyl-terminal region of IE1 is required for this function. We found no defect in the initial events in IFNγ signaling or in nuclear accumulation of signal transducer and activator of transcription 1 STAT1 in IE1-expressing cells. Moreover, we did not observe an association between disruption of IFNγ signaling and nuclear domain 10 ND10 disruption. However, there is reduced binding of STAT1 homodimers to target gamma activated sequence GAS elements in the presence of IE1. Co-immunoprecipitation studies failed to support a direct interaction between IE1 and STAT1, although these studies revealed that the C-terminal region of IE1 was required for interaction with STAT2. Together, these results indicate that IE1 disrupts IFNγ signaling by interfering with signaling events in the nucleus through a novel mechanism. View Full-Text

Keywords: human cytomegalovirus; interferons; interferon g; immediate early 1; IE1; STAT1; STAT2; ND10; antiviral defense human cytomegalovirus; interferons; interferon g; immediate early 1; IE1; STAT1; STAT2; ND10; antiviral defense





Author: Bindu Raghavan †, Charles H. Cook and Joanne Trgovcich *

Source: http://mdpi.com/



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