Development and Application of Quantitative Detection Method for Viral Hemorrhagic Septicemia Virus VHSV Genogroup IVaReport as inadecuate




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1

Department of Aqualife Medicine, Chonnam National University, Yeosu 550-749, Korea

2

Aquatic Life Disease Control Division, National Fisheries Research and Development Institute, Busan 619-902, Korea





*

Author to whom correspondence should be addressed.



Abstract Viral hemorrhagic septicemia virus VHSV is a problematic pathogen in olive flounder Paralichthys olivaceus aquaculture farms in Korea. Thus, it is necessary to develop a rapid and accurate diagnostic method to detect this virus. We developed a quantitative RT-PCR qRT-PCR method based on the nucleocapsid N gene sequence of Korean VHSV isolate Genogroup IVa. The slope and R2 values of the primer set developed in this study were −0.2928 96% efficiency and 0.9979, respectively. Its comparison with viral infectivity calculated by traditional quantifying method TCID50 showed a similar pattern of kinetic changes in vitro and in vivo. The qRT-PCR method reduced detection time compared to that of TCID50, making it a very useful tool for VHSV diagnosis. View Full-Text

Keywords: viral hemorrhagic septicemia virus VHSV; quantitative detection; real-time PCR; diagnostic; olive flounder viral hemorrhagic septicemia virus VHSV; quantitative detection; real-time PCR; diagnostic; olive flounder





Author: Jong-Oh Kim 1, Wi-Sik Kim 1, Si-Woo Kim 1, Hyun-Ja Han 2, Jin Woo Kim 2, Myoung Ae Park 2 and Myung-Joo Oh 1,*

Source: http://mdpi.com/



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