Correlation of ATP Citrate Lyase and Acetyl CoA Levels with Trichothecene Production in Fusarium graminearumReport as inadecuate




Correlation of ATP Citrate Lyase and Acetyl CoA Levels with Trichothecene Production in Fusarium graminearum - Download this document for free, or read online. Document in PDF available to download.

1

Department of Applied Biological Chemistry, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan

2

National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya-ku, Tokyo 158-8501, Japan





*

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Abstract The correlation of ATP citrate lyase ACL and acetyl CoA levels with trichothecene production in Fusarium graminearum was investigated using an inhibitor precocene II and an enhancer cobalt chloride of trichothecene production by changing carbon sources in liquid medium. When precocene II 30 µM was added to inhibit trichothecene production in a trichothecene high-production medium containing sucrose, ACL expression was reduced and ACL mRNA level as well as acetyl CoA amount in the fungal cells were reduced to the levels observed in a trichothecene trace-production medium containing glucose or fructose. The ACL mRNA level was greatly increased by addition of cobalt chloride in the trichothecene high-production medium, but not in the trichothecene trace-production medium. Levels were reduced to those level in the trichothecene trace-production medium by addition of precocene II 300 µM together with cobalt chloride. These results suggest that ACL expression is activated in the presence of sucrose and that acetyl CoA produced by the increased ALC level may be used for trichothecene production in the fungus. These findings also suggest that sucrose is important for the action of cobalt chloride in activating trichothecene production and that precocene II may affect a step down-stream of the target of cobalt chloride. View Full-Text

Keywords: ATP citrate lyase; precocene II; acetyl CoA; trichothecene production; cobalt chloride; sucrose; Fusarium graminearum ATP citrate lyase; precocene II; acetyl CoA; trichothecene production; cobalt chloride; sucrose; Fusarium graminearum





Author: Naoko Sakamoto 1, Rie Tsuyuki 1, Tomoya Yoshinari 2, Jermnak Usuma 1, Tomohiro Furukawa 1, Hiromichi Nagasawa 1 and Shohei Sakuda 1,*

Source: http://mdpi.com/



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