Production of Biodiesel Using Immobilized Lipase and the Characterization of Different Co-Immobilizing Agents and Immobilization MethodsReport as inadecuate


Production of Biodiesel Using Immobilized Lipase and the Characterization of Different Co-Immobilizing Agents and Immobilization Methods


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1

Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China

2

China National Publications Import & Export Group Corporation, Beijing 100020, China

3

Amoy—BUCT Industrial Bio-technovation Institute, Amoy 361022, China

4

State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China





*

Author to whom correspondence should be addressed.



Academic Editor: Michael Wachendorf

Abstract Lipase from Candida sp. 99–125 is widely employed to catalyzed transesterification and can be used for biodiesel production. In this study, the lipase was immobilized by combined adsorption and entrapment to catalyze biodiesel production from waste cooking oil WCO via transesterification, and investigating co-immobilizing agents as additives according to the enzyme activity. The addition of the mixed co-immobilizing agents has positive effects on the activities of the immobilized lipase. Three different immobilizing methods were compared by the conversion ratio of biodiesel and structured by Atom Force Microscopy AFM and Scanning Electron Microscopy SEM, respectively. It was found that entrapment followed by adsorption was the best method. The effect of the co-immobilizing agent amount, lipase dosage, water content, and reuse ability of the immobilized lipase was investigated. By comparison with previous research, this immobilized lipase showed good reuse ability: the conversion ratio excesses 70% after 10 subsequent reactions, in particular, was better than Novozym435 and TLIM on waste cooking oil for one unit of lipase. View Full-Text

Keywords: immobilization; lipase; co-immobilizing agent; biodiesel; biocatalyst immobilization; lipase; co-immobilizing agent; biodiesel; biocatalyst





Author: Kang Zhao 1,2, Qinjian Di 1, Xi Cao 1, Meng Wang 1, Li Deng 1,3,* and Fang Wang 1,4

Source: http://mdpi.com/



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