Synergistic Effects of n-Hexane Fraction of Parkia biglobosa Jacq. Bark Extract and Selected Antibiotics on Bacterial IsolatesReport as inadecuate


Synergistic Effects of n-Hexane Fraction of Parkia biglobosa Jacq. Bark Extract and Selected Antibiotics on Bacterial Isolates


Synergistic Effects of n-Hexane Fraction of Parkia biglobosa Jacq. Bark Extract and Selected Antibiotics on Bacterial Isolates - Download this document for free, or read online. Document in PDF available to download.

1

SAMRC Microbial Water Quality Monitoring Centre, University of Fort Hare, Private Bag X314, Alice 5700, South Africa

2

Applied and Environmental Microbiology Research Group, Department of Biochemistry and Microbiology, University of Fort Hare, Alice 5700, South Africa

3

Department of Microbiology, Obafemi Awolowo University, Ile-Ife 220, Osun State, Nigeria





*

Author to whom correspondence should be addressed.



Academic Editor: Marc A. Rosen

Abstract The incidence of resistance to commonly used antimicrobial agents by microbial pathogens demands increased effort in the development of effective ways of treating infections and diseases. The n-hexane fraction of lyophilized crude bark extract of Parkia biglobosa Jacq. was prepared and, in combination with selected antibiotics, assayed for antimicrobial activity against some selected bacterial pathogens using time-kill assay. Protein leakage analysis of the combined agents was performed using Bradford protein quantification method. Determination of active compounds present in the n-hexane fraction was done using Fourier Transform Infrared Spectroscopy FTIR. While time-kill assay detected 43.33% synergy; 56.67% indifference and no antagonism at 1-2 × minimum inhibitory concentration MIC, 1 × MIC exhibited 55% synergy, 45% indifference and no antagonism. Protein leakages from the cells of selected bacteria ranged from 1.20 µg-mL to 256.93 µg-mL. The presence of a phenyl group, an aromatic ring and phenolic compounds in the n-hexane fraction was confirmed at 2162 cm−1–2020 cm−1, 1605 cm−1–1533 cm−1 and 1438 cm−1–1444 cm−1 spectra peaks, respectively. The observed antibiotic−n-hexane fraction synergistic interaction revealed the improved antibacterial activity of the selected antibiotics. Hence, exploration of a combination of antibiotics with plant secondary metabolites is hereby advocated in the global quest for means of combating infectious diseases caused by multidrug-resistant pathogens. View Full-Text

Keywords: synergy; antimicrobial; pathogens; n-hexane fraction; time-kill assay; protein leakage; Fourier transform infrared spectroscopy FTIR synergy; antimicrobial; pathogens; n-hexane fraction; time-kill assay; protein leakage; Fourier transform infrared spectroscopy FTIR





Author: Oluwatayo E. Abioye 1,2,3,* , David A. Akinpelu 2,3 and Anthony I. Okoh 1,2

Source: http://mdpi.com/



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