Diagnosis of Intoxication by the Organophosphate VX: Comparison Between an Electrochemical Sensor and Ellmans Photometric MethodReport as inadecuate




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Centre of Advanced Studies and Department of Toxicology, Faculty of Military Health Sciences, University of Defense - Trebesska 1575, 50001 Hradec Kralove, Czech Republic





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Abstract An electrochemical sensor is introduced as a tool applicable for diagnosis of intoxication by cholinesterase inhibitors caused by the well-known nerve agent VX. The traditional Ellman method was chosen for comparison with the sensors analytical parameters. Both methods are based on estimation of blood cholinesterase inhibition as a marker of intoxication. While Ellmans method provided a limit of detection of 5.210-7 M for blood containing VX, the electrochemical sensor was able to detect 4.010-7 M. Good correlation between both methods was observed R = 0.92. The electrochemical sensor could be considered a convenient tool for a fast yet accurate method, easily available for field as well as laboratory use. Time and cost savings are key features of the sensor-based assay. View Full-Text

Keywords: Organophosphate; carbamate; intoxication; diagnosis; biosensor Organophosphate; carbamate; intoxication; diagnosis; biosensor





Author: Miroslav Pohanka * , Martina Hrabinova and Kamil Kuca

Source: http://mdpi.com/



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