Immobilization of the Enzyme Glucose Oxidase on Both Bulk and Porous SiO2 SurfacesReport as inadecuate




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1

CNR – IMM Stradale Primosole 50 Catania, Italy

2

Università degli Studi di Catania, Dipartimento di Chimica Biologica, Chimica Medica e Biologia Molecolare, Catania, Italy

3

Università degli Studi di Catania, Dipartimento di Scienze Biomediche, Catania, Italy

4

Laboratorio Superfici e Interfasi SUPERLAB, Consorzio Catania Ricerche, Catania, Italy





*

Author to whom correspondence should be addressed.



Abstract Silicon dioxide surfaces, both bulk and porous, were used to anchor the enzyme glucose oxidase. The immobilization protocol was optimized and the samples characterized using X-ray Photoelectron Spectroscopy, Energy Dispersive X-rays coupled to scanning electron microscopy and enzymatic activity measurements. We show that a uniform layer was obtained by activating the oxide before immobilization. X-ray Photoelectron Spectroscopy measurements carried out on bulk oxide showed that the silicon substrate signal was fully screened after the enzyme deposition showing the absence of uncovered surface regions. The enzyme presence was detected monitoring both the C 1s and N 1s signals. Finally, enzymatic activity measurements confirmed that the glucose oxidase activity was preserved after immobilization and maintained after three months of shelf life if the sample was properly stored. The importance of using porous silicon oxide to maximize the surface area was also evidenced. View Full-Text

Keywords: Glucose oxidase; oxide activation; glutaraldehyde; enzymatic activity; spectrophotometric assay; biosensor Glucose oxidase; oxide activation; glutaraldehyde; enzymatic activity; spectrophotometric assay; biosensor





Author: Sebania Libertino 1,* , Venera Aiello 2,3, Antonino Scandurra 4, Marcella Renis 2 and Fulvia Sinatra 3

Source: http://mdpi.com/



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