Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip SystemReport as inadecuate




Real-time PCR Machine System Modeling and a Systematic Approach for the Robust Design of a Real-time PCR-on-a-Chip System - Download this document for free, or read online. Document in PDF available to download.

Department of Energy and Refrigerating Air Conditioning Engineering, National Taipei University of Technology, Taipei, Taiwan





Abstract Chip-based DNA quantification systems are widespread, and used in many point-of-care applications. However, instruments for such applications may not be maintained or calibrated regularly. Since machine reliability is a key issue for normal operation, this study presents a system model of the real-time Polymerase Chain Reaction PCR machine to analyze the instrument design through numerical experiments. Based on model analysis, a systematic approach was developed to lower the variation of DNA quantification and achieve a robust design for a real-time PCR-on-a-chip system. Accelerated lift testing was adopted to evaluate the reliability of the chip prototype. According to the life test plan, this proposed real-time PCR-on-a-chip system was simulated to work continuously for over three years with similar reproducibility in DNA quantification. This not only shows the robustness of the lab-on-a-chip system, but also verifies the effectiveness of our systematic method for achieving a robust design. View Full-Text

Keywords: DNA quantification reliability; robust design; system identification model; real-time PCR machine; real-time PCR on-a-chip DNA quantification reliability; robust design; system identification model; real-time PCR machine; real-time PCR on-a-chip





Author: Da-Sheng Lee

Source: http://mdpi.com/



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