An Approach to an Inhibition Electronic Tongue to Detect On-Line Organophosphorus Insecticides Using a Computer Controlled Multi-Commuted Flow SystemReport as inadecuate




An Approach to an Inhibition Electronic Tongue to Detect On-Line Organophosphorus Insecticides Using a Computer Controlled Multi-Commuted Flow System - Download this document for free, or read online. Document in PDF available to download.

1

Centro de Investigación y de Estudios Avanzados del Intituto Politecnico Nacional, Av. Instituto Politécnico Nacional 2508, Mexico

2

Université de Perpignan Via Domitia, IMAGES EA4218, Centre de Phytopharmacie, 52 Avenue Paul Alduy, 66860 Perpignan Cedex, France





*

Author to whom correspondence should be addressed.



Abstract An approach to an inhibition bioelectronic tongue is presented. The work is focused on development of an automated flow system to carry out experimental assays, a custom potentiostat to measure the response from an enzymatic biosensor, and an inhibition protocol which allows on-line detections. A Multi-commuted Flow Analysis system MCFA was selected and developed to carry out assays with an improved inhibition method to detect the insecticides chlorpyrifos oxon CPO, chlorfenvinfos CFV and azinphos methyl-oxon AZMO. The system manifold comprised a peristaltic pump, a set of seven electronic valves controlled by a personal computer electronic interface and software based on LabView® to control the sample dilutions into the cell. The inhibition method consists in the injection of the insecticide when the enzyme activity has reached the plateau of the current; with this method the incubation time is avoided. A potentiostat was developed to measure the response from the enzymatic biosensor. Low limits of detection of 10 nM for CPO, CFV, and AZMO were achieved. View Full-Text

Keywords: biosensor; enzyme inhibition; flow system; multi-commuted; organo-phosphorus insecticides; potentiostat biosensor; enzyme inhibition; flow system; multi-commuted; organo-phosphorus insecticides; potentiostat





Author: Gustavo A. Alonso 1,* , Rocio B. Dominguez 1, Jean-Louis Marty 2 and Roberto Muñoz 1

Source: http://mdpi.com/



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