Optimization of Dengue Immunoassay by Label-Free Interferometric Optical Detection MethodReport as inadecuate




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1

Group of Micro-nano Photonics and Biophotonics, Laser Center, and Center for Biomedical Technology, Universidad Politécnica de Madrid, 28040 Madrid, Spain

2

Applied Physics Department, Escuela Técnica Superior de Ingenieros Industriales, Universidad Politécnica de Madrid, José Gutiérrez Abascal, 2, 28006 Madrid, Spain





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Abstract In this communication we report a direct immunoassay for detecting dengue virus by means of a label-free interferometric optical detection method. We also demonstrate how we can optimize this sensing response by adding a blocking step able to significantly enhance the optical sensing response. The blocking reagent used for this optimization is a dry milk diluted in phosphate buffered saline. The recognition curve of dengue virus over the proposed surface sensor demonstrates the capacity of this method to be applied in Point of Care technology. View Full-Text

Keywords: photonic sensing cells; biosensors; label-free immunoassay; dengue virus photonic sensing cells; biosensors; label-free immunoassay; dengue virus





Author: María F. Laguna 1,2,* , Miguel Holgado 1,2, Francisco J. Sanza 1, Alvaro Lavín 1,2, Ana López 1 and Rafael Casquel 1,2

Source: http://mdpi.com/



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