In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds 1-TDPPO Mixed Prop-2-en-1-one with Difluoro PhenylReport as inadecuate


In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds 1-TDPPO Mixed Prop-2-en-1-one with Difluoro Phenyl


In Vitro Studies on a Microfluidic Sensor with Embedded Obstacles Using New Antibacterial Synthetic Compounds 1-TDPPO Mixed Prop-2-en-1-one with Difluoro Phenyl - Download this document for free, or read online. Document in PDF available to download.

1

Biotechnology Research Division, Advanced Radiation Technology Institute ARTI, Korea Atomic Energy Research Institute KAERI, 1266, Sinjeong-Dong, Jeongeup, Jeonbuk 580-185, Korea

2

Department of Textile Engineering and Technology, Yeungnam University, 280 Daehak-ro, Gyeongsan, Gyeongbuk 38541, Korea

3

Department of Chemistry, Maharishi Markandeshwar University, Mullana, Ambala Haryana 133207, India

4

Ministry of Employment and Labor, Major Industrial Accident Prevention Center, 34 Yeosusandallo, Yeosu-Si, Jeonnam 59631, Korea



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Alexander Star

Abstract This paper describes the use of an analytical microfluidic sensor for accelerating chemo-repellent response and strong anti-bacterial 1-Thien-2-yl-3-2, 6-difluoro phenyl prop-2-en-1-one 1-TDPPO. The chemically-synthesized antimicrobial agent, which included prop-2-en-1-one and difluoro phenyl groups, was moving through an optically transparent polydimethylsiloxane PDMS microfluidic sensor with circular obstacles arranged evenly. The response, growth and distribution of fluorescent labeling Pseudomonas aeruginosa PAO1 against the antimicrobial agent were monitored by confocal laser scanning microscope CLSM. The microfluidic sensor along with 1-TDPPOin this study exhibits the following advantages: i Real-time chemo-repellent responses of cell dynamics; ii Rapid eradication of biofilm by embedded obstacles and powerful antibacterial agents, which significantly reduce the response time compared to classical methods; iii Minimal consumption of cells and antimicrobial agents; and iv Simplifying the process of the normalization of the fluorescence intensity and monitoring of biofilm by captured images and datasets. View Full-Text

Keywords: PDMS microfluidic sensor; confocal laser scanning microscope; 1-TDPPO; Pseudomonas aeruginosa PAO1; fluorescence intensity PDMS microfluidic sensor; confocal laser scanning microscope; 1-TDPPO; Pseudomonas aeruginosa PAO1; fluorescence intensity





Author: Changhyun Roh 1,†, Jaewoong Lee 2,†, Mayank Kinger 3 and Chankyu Kang 4,*

Source: http://mdpi.com/



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