Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real SamplesReport as inadecuate


Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples


Comparison between a Direct-Flow SPR Immunosensor for Ampicillin and a Competitive Conventional Amperometric Device: Analytical Features and Possible Applications to Real Samples - Download this document for free, or read online. Document in PDF available to download.

1

Department of Chemistry -Sapienza- University of Rome, P.le A. Moro, 5, 00185 Rome, Italy

2

Department of Chemistry and Pharmacy Technology -Sapienza- University of Rome, P.le A. Moro, 5, 00185 Rome, Italy





*

Author to whom correspondence should be addressed.



Abstract In this research, we developed a direct-flow surface plasmon resonance SPR immunosensor for ampicillin to perform direct, simple, and fast measurements of this important antibiotic. In order to better evaluate the performance, it was compared with a conventional amperometric immunosensor, working with a competitive format with the aim of finding out experimental real advantages and disadvantages of two respective methods. Results showed that certain analytical features of the new SPR immunodevice, such as the lower limit of detection LOD value and the width of the linear range, are poorer than those of a conventional amperometric immunosensor, which adversely affects the application to samples such as natural waters. On the other hand, the SPR immunosensor was more selective to ampicillin, and measurements were more easily and quickly attained compared to those performed with the conventional competitive immunosensor. View Full-Text

Keywords: ampicillin; flow surface plasmon resonance SPR immunosensor; competitive amperometric immunosensor; analytical comparison ampicillin; flow surface plasmon resonance SPR immunosensor; competitive amperometric immunosensor; analytical comparison





Author: Mauro Tomassetti 1,* , Giovanni Merola 1, Elisabetta Martini 1, Luigi Campanella 1, Gabriella Sanzò 2, Gabriele Favero 2 and Franco Mazzei 2

Source: http://mdpi.com/



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