Preparation and Biological Evaluation of a 55Co-2-Acetylpyridine ThiosemicarbazoneReport as inadecuate




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Agricultural, Medical and Industrial Research School AMIRS, Nuclear Science and Technology Research Institute NSTRI Karaj, P.O.Box: 31485-498, Iran





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Abstract Due to the anti-proliferative properties of cobalt-thiosemicarbazone complexes, the production of 55CoIII-bis-2-acetylpyridine thiosemicarbazone 55CoIIIAPTS2 was investigated. Co-55 T1-2=17.53 h was produced by 150 μA irradiation of a natural nickel target by 15 MeV protons. The 55Co was separated from the irradiated target material using a two-step method with a radiochemical yield of >95% followed by radionuclidic and chemical purity control. 55CoIIIchloride was mixed with 2-acetylpyridine thiosemicarbazone for 30 min at room temperature to yield 55CoIIIAPTS2 radiochemical purity > 98% shown by RTLC-HPLC. A specific activity of about 10–20 Ci-mmol was obtained. The final solution was diluted in normal saline to 5% ethanolic solution for biological evaluation. The stability of the final product was checked in the absence and presence of human serum at 37°C to 24 h. The partition co-efficient of the final complex at the pH of 7 was 1.00±0.08. A significant tumor accumulation %ID-g; 3.5% was observed in tumoral tissue 21 h post injection in fibrosarcoma-bearing mice by biodistribution studies. Co-incidence imaging also demonstrated tumor uptake from 21–35 h however at 35 h tumor uptake is more specific and significant.

Keywords: Cobalt-55; Thiosemicarbazone; Biodistribution; Tumor imaging; Co-incidence; Fibrosarcoma Cobalt-55; Thiosemicarbazone; Biodistribution; Tumor imaging; Co-incidence; Fibrosarcoma





Author: Amir R. JALILIAN * , Pejman ROWSHANFARZAD, Mehdi AKHLAGHI, Mahsheed SABET, Mohsen KAMALI-DEHGHAN and Mehrban POULADI

Source: http://mdpi.com/



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