An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew Drosera rotundifolia L.Report as inadecuate




An Improved 2-step Liquid Culture System for Efficient In Vitro Shoot Proliferation of Sundew Drosera rotundifolia L. - Download this document for free, or read online. Document in PDF available to download.

Department of Pharmacognosy, University of Vienna, Althanstr. 14, A-1090, Vienna, Austria





*

Author to whom correspondence should be addressed.



Abstract An efficient procedure for in vitro shoot production of the medicinal plant sundew Drosera rotundifolia L. was developed. Of three cytokinins tested, zeatin ZEA at a concentration of 2 μM resulted in the formation of large numbers of adventitious shoots on leaf explants. A larger size of the small shoots was achieved by combining a 2-weeks preculture with ZEA step 1: shoot induction with a 5-weeks main culture without plant growth regulators step 2: shoot elongation. Liquid media were superior to semisolid media: An average of 27.4 shoots per leaf explant, and 53.3 shoots per shoot explant were achieved. The 2-step culture system with liquid media permits a comparatively cheap micropropagation of sundew as well as in vitro biomass production, with potential for scale-up.

Keywords: Endangered; Medicinal Plant; Liquid Medium; Micropropagation Endangered; Medicinal Plant; Liquid Medium; Micropropagation





Author: Christoph WAWROSCH * , Eva BENDA and Brigitte KOPP

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents