Assessment of est-ssr markers for genetic analysis on coffee Report as inadecuate




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EVELINE TEIXEIRA CAIXETA ; EUNIZE MACIEL ZAMBOLIM ; GUILHERME FERREIRA PENA ; ANA PAULA RIBEIRO ; LAÉRCIO ZAMBOLIM ; ANTÔNIO ALVES PEREIRA ; NEY SUSSUMU SAKIYAMA ;Bragantia 2009, 68 3

Author: ROBSON FERNANDO MISSIO

Source: http://www.redalyc.org/


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Bragantia ISSN: 0006-8705 editor@iac.sp.gov.br Instituto Agronômico de Campinas Brasil MISSIO, ROBSON FERNANDO; TEIXEIRA CAIXETA, EVELINE; ZAMBOLIM, EUNIZE MACIEL; FERREIRA PENA, GUILHERME; RIBEIRO, ANA PAULA; ZAMBOLIM, LAÉRCIO; ALVES PEREIRA, ANTÔNIO; SUSSUMU SAKIYAMA, NEY ASSESSMENT OF EST-SSR MARKERS FOR GENETIC ANALYSIS ON COFFEE Bragantia, vol.
68, núm.
3, 2009, pp.
573-581 Instituto Agronômico de Campinas Campinas, Brasil Available in: http:--www.redalyc.org-articulo.oa?id=90811757003 How to cite Complete issue More information about this article Journals homepage in redalyc.org Scientific Information System Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Non-profit academic project, developed under the open access initiative EST-SSR markers for genetic analysis of Coffea 573 ASSESSMENT OF EST-SSR MARKERS FOR GENETIC ANALYSIS ON COFFEE ( 1) ROBSON FERNANDO MISSIO (2); EVELINE TEIXEIRA CAIXETA (2,3*); EUNIZE MACIEL ZAMBOLIM (2); GUILHERME FERREIRA PENA (2); ANA PAULA RIBEIRO (2); LAÉRCIO ZAMBOLIM (2); ANTÔNIO ALVES PEREIRA (4); NEY SUSSUMU SAKIYAMA (2) ABSTRACT EST-SSR markers were used to investigate the genetic diversity among and within coffee populations, to explore the possibility of their use for fingerprinting of cultivars and to assist breeding programs.
Seventeen markers, developed from ESTs (Expressed Sequence Tags) from the Brazilian Coffee Genome Project, were used.
All markers showed polymorphism among the genotypes assessed.
The average number of allele per primer was 5.1.
The highest polymorphisms were found within C.
canephora (88.2%) and rust-resistant varieties (35.3%).
About 29.4% of the markers differentiated C.
arabica from Híbrido de Timor; it was also possible to identify those closest and farthest from C.
arabica.
The analysis of population-grouped genotypes revealed a 64.0% genetic diversity among and a 36.0% genetic diversity within populations.
The differentiation index was 0.637...





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