Metabolomic Profiling of Soybeans Glycine max L. Reveals the Importance of Sugar and Nitrogen Metabolism under Drought and Heat StressReport as inadecuate


Metabolomic Profiling of Soybeans Glycine max L. Reveals the Importance of Sugar and Nitrogen Metabolism under Drought and Heat Stress


Metabolomic Profiling of Soybeans Glycine max L. Reveals the Importance of Sugar and Nitrogen Metabolism under Drought and Heat Stress - Download this document for free, or read online. Document in PDF available to download.

1

Department of Biology and Microbiology, South Dakota State University, Brookings, SD 57007, USA

2

Department of Plant Biology, University of Vermont, Burlington, VT 05405, USA

3

22nd Century Group Inc., Clarence, NY 14031, USA

4

Dale Bumpers National Rice Research Center, USDA-ARS, Stuttgart, AR 72160, USA





*

Author to whom correspondence should be addressed.



Academic Editors: Masayuki Fujita and Mirza Hasanuzzaman

Abstract Soybean is an important crop that is continually threatened by abiotic stresses, especially drought and heat stress. At molecular levels, reduced yields due to drought and heat stress can be seen as a result of alterations in metabolic homeostasis of vegetative tissues. At present an incomplete understanding of abiotic stress-associated metabolism and identification of associated metabolites remains a major gap in soybean stress research. A study with a goal to profile leaf metabolites under control conditions 28-24 °C, drought 28-24 °C, 10% volumetric water content VWC, and heat stress 43-35 °C was conducted in a controlled environment. Analyses of non-targeted metabolomic data showed that in response to drought and heat stress, key metabolites carbohydrates, amino acids, lipids, cofactors, nucleotides, peptides and secondary metabolites were differentially accumulated in soybean leaves. The metabolites for various cellular processes, such as glycolysis, the tricarboxylic acid TCA cycle, the pentose phosphate pathway, and starch biosynthesis, that regulate carbohydrate metabolism, amino acid metabolism, peptide metabolism, and purine and pyrimidine biosynthesis, were found to be affected by drought as well as heat stress. Computationally based regulatory networks predicted additional compounds that address the possibility of other metabolites and metabolic pathways that could also be important for soybean under drought and heat stress conditions. Metabolomic profiling demonstrated that in soybeans, keeping up with sugar and nitrogen metabolism is of prime significance, along with phytochemical metabolism under drought and heat stress conditions. View Full-Text

Keywords: crop yield; drought stress; heat stress; metabolomic profiling; phytochemicals; soybean crop yield; drought stress; heat stress; metabolomic profiling; phytochemicals; soybean





Author: Aayudh Das 1,2, Paul J. Rushton 1,3 and Jai S. Rohila 1,4,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents