Fluorescent β-Blockers as Tools to Study Presynaptic Mechanisms of NeurosecretionReport as inadecuate




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1

Institute of BioOrganic -Antonio Gonzalez-, University of La Laguna, La Laguna 38071, Tenerife, Spain

2

Institute de Natural Products and Agrobiology, Spanish National Research Council CSIC, La Laguna 38071, Tenerife, Spain

3

Unit of Pharmacology, Medical School, University of La Laguna, La Laguna 38071, Tenerife, Spain





*

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Abstract Several, if not all adrenergic β-blockers β-Bs, accumulate progressively inside secretory vesicles in a time- and concentration-dependent manner, and could be considered to be false neurotransmitters. This transmitter effect is most likely unrelated to their ability to block adrenergic receptors, but it could explain the delay in lowering arterial pressure in hypertensive patients. We have developed a new drug to monitor the accumulation of β-Bs inside living cells, RCTM-3, which fluoresces in the visible spectrum. Here we describe the procedure to synthesize this new compound, as well as its fluorescent properties, pharmacological profile and its accumulation inside the secretory vesicles of PC12 cells. View Full-Text

Keywords: exocytosis; false neurotransmitter; secretory vesicle; TIRF exocytosis; false neurotransmitter; secretory vesicle; TIRF





Author: Beatriz Beltran 1, Romen Carrillo 2, Tomas Martin 1,2, Victor S. Martin 1, Jose D. Machado 3 and Ricardo Borges 1,3,*

Source: http://mdpi.com/



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