Benefits of Structured and Free Monoacylglycerols to Deliver Eicosapentaenoic EPA in a Model of Lipid MalabsorptionReport as inadecuate




Benefits of Structured and Free Monoacylglycerols to Deliver Eicosapentaenoic EPA in a Model of Lipid Malabsorption - Download this document for free, or read online. Document in PDF available to download.

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Abstract In the present study, we used a preclinical model of induced lipolytic enzyme insufficiency, and hypothesized that the use of monoacylglycerols MAG will enhance their bioavailability and delivery to the tissues. Experimental diets containing 20% lipids were fed to rats for 21 days with or without Orlistat. The control diet of fish oil FO, a source of EPA and DHA, was tested against: structured A vanillin acetal of sn-2 MAG Vanil + O and B diacetyl derivative of sn-2 MAG Acetyl + O and C free MAG MAG + O. FA profiles with an emphasis on EPA and DHA levels were determined in plasma, red blood cells RBC, liver, spleen, brain and retina. We observed significant reduction of lipid absorption when rats co-consumed Orlistat. As expected, the FO groups with and without Orlistat showed the biggest difference. The Vanil + O, Acetyl + O and MAG + O groups, demonstrated higher levels of EPA 5.5 ± 1.9, 4.6 ± 1.6 and 5.6 ± 0.6, respectively in RBC compared with FO + O diets 3.3 ± 0.2, 2.6 ± 0.2. Levels of EPA incorporation, in plasma, were similar to those obtained for RBC, and similar trends were observed for the collected tissues and even with DHA levels. These observations with two MAG derivatives providing the fatty acid esterified in the sn-2 position, show that these molecules are efficient vehicles of EPA in malabsorption conditions which is in line with our hypothesis. Free MAG, characterized as having exclusively sn-13 isomers of EPA, demonstrated better absorption efficiencies and accretion to tissues when compared to structured MAG. The study demonstrated that structured and free MAG can be used efficiently as an enteral vehicle to supply bioactive fatty acids such as EPA and DHA in lipid malabsorption where diminished lipolytic activity is the underlying cause. View Full-Text

Keywords: malabsorption; monoacylglycerol; long-chain polyunsaturated fatty acids; eicosapentaenoic acid; docosahexaenoic acid malabsorption; monoacylglycerol; long-chain polyunsaturated fatty acids; eicosapentaenoic acid; docosahexaenoic acid





Author: Cristina Cruz-Hernandez * , Sagar K. Thakkar, Julie Moulin, Manuel Oliveira, Isabelle Masserey-Elmelegy, Fabiola Dionisi and Frédéric Destaillats

Source: http://mdpi.com/



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