The Extract of Aster Koraiensis Prevents Retinal Pericyte Apoptosis in Diabetic Rats and Its Active Compound, Chlorogenic Acid Inhibits AGE Formation and AGE-RAGE InteractionReport as inadecuate


The Extract of Aster Koraiensis Prevents Retinal Pericyte Apoptosis in Diabetic Rats and Its Active Compound, Chlorogenic Acid Inhibits AGE Formation and AGE-RAGE Interaction


The Extract of Aster Koraiensis Prevents Retinal Pericyte Apoptosis in Diabetic Rats and Its Active Compound, Chlorogenic Acid Inhibits AGE Formation and AGE-RAGE Interaction - Download this document for free, or read online. Document in PDF available to download.

Korean Medicine Convergence Research Division, Korea Institute of Oriental Medicine, Daejeon 34054, Korea





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Abstract Retinal capillary cell loss is a hallmark of early diabetic retinal changes. Advanced glycation end products AGEs are believed to contribute to retinal microvascular cell loss in diabetic retinopathy. In this study, the protective effects of Aster koraiensis extract AKE against damage to retinal vascular cells were investigated in streptozotocin STZ-induced diabetic rats. To examine this issue further, AGE accumulation, nuclear factor-kappaB NF-κB and inducible nitric oxide synthase iNOS were investigated using retinal trypsin digests from streptozotocin-induced diabetic rats. In the diabetic rats, TUNEL Terminal deoxynucleotidyl transferase mediated dUTP Nick End Labeling-positive retinal microvascular cells were markedly increased. Immunohistochemical studies revealed that AGEs were accumulated within the retinal microvascular cells, and this accumulation paralleled the activation of NF-κB and the expression of iNOS in the diabetic rats. However, AKE prevented retinal microvascular cell apoptosis through the inhibition of AGE accumulation and NF-κB activation. Moreover, to determine the active compounds of AKE, two major compounds, chlorogenic acid and 3,5-di-O-caffeoylquinic acid, were tested in an in vitro assay. Among these compounds, chlorogenic acid significantly reduced AGE formation as well as AGE-RAGE receptor for AGEs binding activity. These results suggest that AKE, particularly chlorogenic acid, is useful in inhibiting AGE accumulation in retinal vessels and exerts a preventive effect against the injuries of diabetic retinal vascular cells. View Full-Text

Keywords: advanced glycation end products; diabetic retinopathy; Aster koraiensis advanced glycation end products; diabetic retinopathy; Aster koraiensis





Author: Junghyun Kim, Kyuhyung Jo, Ik-Soo Lee, Chan-Sik Kim * and Jin Sook Kim *

Source: http://mdpi.com/



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