An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution ProtocolsReport as inadecuate




An Efficient Synthetic Strategy for the Preparation of Nucleic Acid-Encoded Peptide and Protein Libraries for In Vitro Evolution Protocols - Download this document for free, or read online. Document in PDF available to download.

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Abstract We describe an improved synthetic strategy for the preparation of nucleic acid encoded peptide and protein libraries. A solid-phase format was used to prepare and purify a novel type of mRNA-template for in vitro mRNA-protein fusion synthesis. The present protocol simplifies and accelerates the preparation of fusion libraries and should prove most useful for in vitro protein evolution procedures which involve repetitive cycles of fusion library preparation and selection.

Keywords: Nucleic acid-encoded libraries; mRNA-protein fusion; PROfusionTM; in vitro selection; puromycin; psoralen Nucleic acid-encoded libraries; mRNA-protein fusion; PROfusionTM; in vitro selection; puromycin; psoralen





Author: Markus Kurz, Guannan Kuang and Peter A. Lohse *

Source: http://mdpi.com/



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