Relaxant Effect of the Ethanol Extract of Helichrysum plicatum Asteraceae on Isolated Rat Ileum ContractionsReport as inadecuate




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1

Institute for Medicinal Plants Research, Tadeuša Košćuška 1, 11000 Belgrade, Serbia

2

Department of Physiology, Faculty of Medicine, University of Nis, Bulevar dr Zorana Djindjica 81, 18000 Nis, Serbia

3

Department of Pharmacology, Faculty of Medicine, University of Nis, Bulevar dr Zorana Djindjica 81, 18000 Nis, Serbia





*

Author to whom correspondence should be addressed.



Abstract Helichrysum plicatum Turkish Helichrysum has been used in folk medicine for the treatment of gastric and hepatic disorders. The aim of the present study was to examine the relaxant activity of an extract of H. plicatum flowers on isolated rat ileum. Segments of ileum of rats were suspended in an organ bath. Cumulative concentrations of H. plicatum ethanol extract induced a relaxant effect on spontaneous rat ileum contractions. H. plicatum extract caused a mean contractile response of 81.68 ± 6.17% at a dose of 0.01 mg-mL and 30.08 ± 9.07% at a dose of 1 mg-mL. A similar effect was observed with papaverine 0.01–3 μg-mL. H. plicatum extract 0.01–1 mg-mL relaxed high K+ 80 mM precontractions, an effect similar to that caused by papaverine 0.01–3 μg-mL. The plant extract 0.03–0.3 mg-mL also induced a significant depression of the cumulative concentration response curve for acetylcholine 5–1500 nM p < 0.01. Atropine 140 nM abolished the acetylcholine effect. The extract 0.03–0.3 mg-mL reduced the histamine 1–300 nM and BaCl2 3–900 μM induced contractions p < 0.01. Our results showed the relaxant effect of the ethanol extract of Helichrysum plicatum flowers on the isolated rat intestine Extract of H. plicatum can inhibit the spontaneous ileum contractions and contractions induced by acetylcholine, histamine, barium and potassium ions.

Keywords: spasmolytic; Helichrysum plicatum; ileum; rats spasmolytic; Helichrysum plicatum; ileum; rats





Author: Dubravka Bigovic 1, Suzana Brankovic 2, Dusanka Kitic 3,* , Mirjana Radenkovic 2, Teodora Jankovic 1, Katarina Savikin 1 and Slavoljub Zivanovic 3

Source: http://mdpi.com/



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