Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in MiceReport as inadecuate




Optimization of Alkaline Extraction of Polysaccharides from Ganoderma lucidum and Their Effect on Immune Function in Mice - Download this document for free, or read online. Document in PDF available to download.

1

College of Light Industry and Food Sciences, South China University of Technology, Guangzhou 510640, China

2

Infinitus China Co., Ltd., Jiangmen 529156, China

3

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China

4

Guangzhou University of Traditional Chinese Medicine, Guangzhou 510632, China





*

Author to whom correspondence should be addressed.



Abstract Response surface methodology was employed to optimize the conditions for alkaline extraction of polysaccharides from Ganoderma lucidum. The results indicated that the optimum conditions were an extraction temperature of 60.1 ºC, an extraction time of 77.3 min, a sodium hydroxide NaOH concentration of 5.1% and a substrate-liquid ratio of 1:21.4. Immunological assays results have shown that the alkaline soluble polysaccharides have no noticeable effects on monocyte phagocytosis and immune organ spleen, thymus weight of of immunocompromised mice at the tested dosages. However, they could restore delayed type hypersensitivity reaction to dinitrofluorobenzene DFNB, hemolysis antibody levels at the three doses applied, and improve the natural killer cell activity at the high-dose and medium dose.

Keywords: Ganoderma lucidum; alkaline extraction; polysaccharides; yield; immune activity Ganoderma lucidum; alkaline extraction; polysaccharides; yield; immune activity





Author: Sheng-Quan Huang 1,2, Jin-Wei Li 3, Zhou Wang 1, Hua-Xin Pan 4, Jiang-Xu Chen 1 and Zheng-Xiang Ning 1,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents