Antibacterial Activity of Aristolochia brevipes against Multidrug-Resistant Mycobacterium tuberculosisReport as inadecuate




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1

Laboratory of Microbiology, Biomedical Research Center of the South IMSS, Argentina 1, Col. Centro, 62790 Xochitepec, Morelos, Mexico

2

Immunochemistry Laboratory II, Department of Immunology, National School of Sciences Bilogy IPN, Prolongación Carpio y Plan de Ayala S-N, Col. Casco de Santo Tomás, 11340 Mexico, D.F., Mexico

3

Chemical Research Center, University of Morelos UAEM, Av. Universidad 1001, Col. Chamilpa, 62209 Cuernavaca, Morelos, Mexico





*

Author to whom correspondence should be addressed.



Abstract The increased incidence of Multidrug-Resistant Mycobacterium tuberculosis MDR-MT requires the search for alternative antimycobacterial drugs. The main aim of this study was to evaluate the dichloromethane extract from Aristolochia brevipes Rhizoma and the compounds isolated from this extract against several mycobacterial strains, sensitive, resistant monoresistant, and clinical isolates multidrug-resistant, using the alamarBlue™ microassay. The extract was fractionated by column chromatography, yielding the following eight major compounds: 1 6α-7-dehydro-N-formylnornantenine; 2 E-Z-N-formylnornantenine; 3 7,9-dimethoxytariacuripyrone; 4 9-methoxy-tariacuripyrone; 5 aristololactam I; 6 β-sitosterol; 7 stigmasterol; and 8 3-hydroxy-α-terpineol. The structures of these compounds were elucidated by 1H- and 13C- 1D and 2D Nuclear Magnetic Resonance NMR spectroscopy. This study demonstrates that the dichloromethane extract rhizome of A. brevipes possesses strong in vitro antimycobacterial activity against Mycobacterium tuberculosis H37Rv Minimum Inhibitory Concentration value MIC, 12.5 µg-mL. The most active compound against all mycobacterial strains tested was the compound aristolactam I 5, with MIC values ranging between 12.5 and 25 µg-mL. To our knowledge, this the first report of antimycobacterial activity in this plant.

Keywords: Mycobacterium tuberculosis; Multidrug-resistant MDR; Aristolochia brevipes; alamarBlue™ microassay; medicinal plant Mycobacterium tuberculosis; Multidrug-resistant MDR; Aristolochia brevipes; alamarBlue™ microassay; medicinal plant





Author: Víctor Manuel Navarro-García 1,* , Julieta Luna-Herrera 2, Ma. Gabriela Rojas-Bribiesca 1, Patricia Álvarez-Fitz 1 and María Yolanda Ríos 3

Source: http://mdpi.com/



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