Application of Preparative High-Speed Counter-Current Chromatography for the Separation of Two Alkaloids from the Roots of Tabernaemontana catharinensis ApocynaceaeReport as inadecuate




Application of Preparative High-Speed Counter-Current Chromatography for the Separation of Two Alkaloids from the Roots of Tabernaemontana catharinensis Apocynaceae - Download this document for free, or read online. Document in PDF available to download.

Laboratório de Ciências Químicas, Universidade Estadual do Norte Fluminense Darcy Ribeiro, 28013-602, Campos dos Goytacazes, Rio de Janeiro, Brazil





*

Author to whom correspondence should be addressed.



Abstract The methanolic extract of Tabernaemontana catharinensis Apocynaceae roots, which contains alkaloids with several biological activities, was separated on a preparative scale using high-speed counter-current chromatography. The optimum solvent system was found to be a mixture of CHCl3-MeOH-H2O 5:10:6 v-v-v and led to a successful separation of two monoterpenic indole alkaloids, voachalotine 1 and 12-methoxy-Nb-methylvoachalotine 2 in approximately 4.0 hours. The alkaloids were all isolated at purities over 95%, and their structures were established on the basis of spectroscopic methods, including 1D and 2D NMR and EI-MS.

Keywords: counter-current chromatography; Tabernaemontana catharinensis; alkaloids counter-current chromatography; Tabernaemontana catharinensis; alkaloids





Author: Milena S. Gonçalves, Ivo J. Curcino Vieira * , Rodrigo R. Oliveira and Raimundo Braz-Filho

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents