Chemical, Antioxidant and Antimicrobial Investigations of Pinus cembra L. Bark and NeedlesReport as inadecuate




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Department of Plant and Animal Biology, School of Pharmacy, Gr. T. Popa University of Medicine and Pharmacy, Universitatii Street Number 16, 700115 Iasi, Romania

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Department of Microbiology, School of Medicine, Gr. T. Popa University of Medicine and Pharmacy, Universitatii Street Number 16, 700115 Iasi, Romania

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Department of Pharmacognosy, School of Pharmacy, Gr. T. Popa University of Medicine and Pharmacy, Universitatii Street Number 16, 700115 Iasi, Romania

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Institute of Biological Research, Lascar Catargi Street Number 47, 700107 Iasi, Romania

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Department of Pharmaceutical Chemistry, School of Pharmacy, University of Oslo, P.O. 1068, Blindern, 0316 Oslo, Norway





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Author to whom correspondence should be addressed.



Abstract The chemical constituents and biological activity of Pinus cembra L. Pinaceae, native to the Central European Alps and the Carpathian Mountains, are not well known. The aim of the present work was to examine the phenolic content, antioxidant and antimicrobial effects of hydromethanolic extracts of Pinus cembra L. bark and needles. Bark extract had higher concentrations of total phenolics 299.3 vs. 78.22 mg gallic acid equivalents-g extract, flavonoids 125.3 vs. 19.84 mg catechin equivalents-g extract and proanthocyanidins 74.3 vs. 12.7 mg cyanidin equivalents-g extract than needle extract and was more active as a free radical scavenger, reducing agent and antimicrobial agent. The EC50 values in the 2,2-diphenyl-1-picrylhydrazyl DPPH, 2,2-azino-bis3-ethylbenzo-thiazoline-6-sulfonic acid diammonium salt ABTS and reducing power assays were 71.1, 6.3 and 26 mg-mL for bark extract and 186.1, 24 and 104 mg-mL for needle extract, respectively. In addition, needle extract showed ferrous ions chelating effects EC50 = 1,755 μg-mL. The antimicrobial effects against Staphylococcus aureus, Sarcina lutea, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Candida albicans were assessed by the agar diffusion method. Both extracts 4 mg-well were active against all the microorganisms tested; bark extract showed higher inhibition on all strains. These results indicate that Pinus cembra L. bark and needles are good sources of phytochemicals with antioxidant and antimicrobial activities.

Keywords: Pinus cembra L.; polyphenols; antioxidant activity; antimicrobial activity Pinus cembra L.; polyphenols; antioxidant activity; antimicrobial activity





Author: Cristina Lungu Apetrei 1, Cristina Tuchilus 2, Ana Clara Aprotosoaie 3, Adrian Oprea 4, Karl Egil Malterud 5 and Anca Miron 3,*

Source: http://mdpi.com/



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