Camptothecin-20s-O-N-3’α,12’α-dihydroxy-24’-carbonyl-5’β-cholan-lysine, a Novel Camptothecin Analogue, Induces Apoptosis towards Hepatocellular Carcinoma SMMC-7721 CellsReport as inadecuate




Camptothecin-20s-O-N-3’α,12’α-dihydroxy-24’-carbonyl-5’β-cholan-lysine, a Novel Camptothecin Analogue, Induces Apoptosis towards Hepatocellular Carcinoma SMMC-7721 Cells - Download this document for free, or read online. Document in PDF available to download.

The Key Laboratory of Forest Plant Ecology, Northeast Forestry University, Ministry of Education, Harbin 150040, China





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Abstract Camptothecin-20s-O-N-3’α,12’α-dihydroxy-24’-carbonyl-5’β-cholan-lysine B2 is a novel camptothecin analogue. Our previous study had shown that it displayed higher cytoxicity activity towards hepatocellular carcinoma SMMC-7721 cells than camptothecin CPT in vitro. In this paper, the underlying mechanism of anti-proliferation of B2 towards SMMC-7721 cells was further examined. Cell growth inhibition of B2 was determined using the 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT assay; morphological changes were observed under Laser Scanning Confocal Microscope LSCM; cell cycle distribution, apoptotic population, changes in mitochondrial membrane potential, intracellular calcium concentration and reactive oxygen species ROS production were determined by flow cytometry FCM. Activities of caspase-3 and caspase-9 were measured, and the expression level of Bcl-2 and Bax proteins were analyzed by Western blot. The results suggested that B2 inhibited SMMC-7721 cell growth by causing cell cycle arrest at the S and G2-M phases, and induced apoptosis involving a mitochondrial pathway. B2 appears to cause a high induction of apoptosis on SMMC-7721 cells in vitro, which suggests it might be a potential drug for cancer therapy.

Keywords: camptothecin analogue; apoptosis; hepatocellular carcinoma SMMC-7721 cell camptothecin analogue; apoptosis; hepatocellular carcinoma SMMC-7721 cell





Author: Qingyong Li * , Wei Qiu, Qiaochu Zhu, Yuangang Zu, Xiaoqiu Deng, Tengfei Zhao, Chunfei Jiang and Li Zhang

Source: http://mdpi.com/



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