Recombinant VAA-I from Viscum album Induces Apoptotic Cell Death of Hepatocellular Carcinoma SMMC7721 CellsReport as inadecuate




Recombinant VAA-I from Viscum album Induces Apoptotic Cell Death of Hepatocellular Carcinoma SMMC7721 Cells - Download this document for free, or read online. Document in PDF available to download.

1

Department of Hepatobiliary and Pancreatic Surgery, the First Clinical Hospital, Jilin University, Jilin 130021, China

2

Department of General Surgery, the Affiliated Hospital, Beihua University, Jilin 132000, China

3

The College of Pharmacy, Beihua University, Jilin 132013, China





*

Author to whom correspondence should be addressed.



Abstract Researchers have proposed that VAA-I, a specific plant lectin found in Viscum album, has therapeutic effects on cancer and autoimmune diseases. VAA-I has shown some promising treatment results in some types of tumor cell lines, especially SMMC-7721 cells human hepatocellular carcinoma cells. However, few details are known about the mechanism and process of cell death induced by VAA-I in tumor cells. In this study, the cell morphology results showed that SMMC-7721 cells treated with VAA-I exhibited several features typical of apoptotic cell death, which was confirmed by the Caspase inhibition assay. Fluo-3-acetoxymethyl ester AM fluorescence imaging techniques showed that rVAA-I significantly elevated the intracellular calcium level Ca2+i in SMMC-7721 cells. These findings suggest that apoptosis may play the most important role in SMMC-7721 cell death induced by rVAA-I. Finally, in the SMMC-7721 cells treated with rVAA-I, a series of genes in the p38 mitogen-activated protein kinase MAPK signaling pathway were expressed differentially, and further found that PI 3-kinase pathway is involved in rVAA-I signal transduction in SMMC-7721 cells.

Keywords: Viscum album; rVAA-I; apoptosis; phosphoinositide 3-kinase Viscum album; rVAA-I; apoptosis; phosphoinositide 3-kinase





Author: Xueliang Yang 1,2, Shuang Jiang 3, Yahui Liu 1, Ping Zhang 1, Shuli Xie 1 and Guangyi Wang 1,*

Source: http://mdpi.com/



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