The Expanding Role of Electrospray Ionization Mass Spectrometry for Probing Reactive Intermediates in SolutionReport as inadecuate




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1

Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministry of Education of China, Hunan Normal University, 36 Lushan Road, Changsha 410081, China

2

School of Pharmaceutical Sciences, Central South University, 172 Tongzipo Road, Changsha 410013, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Abstract Within the past decade, electrospray ionization mass spectrometry ESI-MS has rapidly occupied a prominent position for liquid-phase mechanistic studies due to its intrinsic advantages allowing for efficient -fishing- rapid, sensitive, specific and simultaneous detection-identification of multiple intermediates and products directly from a -real-world- solution. In this review we attempt to offer a comprehensive overview of the ESI-MS-based methodologies and strategies developed up to date to study reactive species in reaction solutions. A full description of general issues involved with probing reacting species from complex biochemical reaction systems is briefly covered, including the potential sources of reactive intermediate metabolite generation, analytical aspects and challenges, basic rudiments of ESI-MS and the state-of-the-art technology. The main purpose of the present review is to highlight the utility of ESI-MS and its expanding role in probing reactive intermediates from various reactions in solution, with special focus on current progress in ESI-MS-based approaches for improving throughput, testing reality and real-time detection by using newly developed MS instruments and emerging ionization sources such as ambient ESI techniques. In addition, the limitations of modern ESI-MS in detecting intermediates in organic reactions is also discussed.

Keywords: intermediates; reactive metabolite; electrospray; ambient ionization; mass spectrometry; chemical trapping; solution intermediates; reactive metabolite; electrospray; ambient ionization; mass spectrometry; chemical trapping; solution





Author: Weitao Zhu 1,†, Yu Yuan 2,†, Peng Zhou 1, Le Zeng 1, Hua Wang 1, Ling Tang 1, Bin Guo 1,* and Bo Chen 1

Source: http://mdpi.com/



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