Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate SubstratesReport as inadecuate




Protein Adsorption on Various Plasma-Treated Polyethylene Terephthalate Substrates - Download this document for free, or read online. Document in PDF available to download.

1

Jozef Stefan International Postgraduate School, Jamova 39, Ljubljana 1000, Slovenia

2

Anti Doping Laboratory Qatar, Doha, 27775, State of Qatar

3

Rudjer Boskovic Institute, Bijenicka 54, Zagreb 10000, Croatia

4

Plasma Laboratory, Institute Jozef Stefan, Jamova 39, Ljubljana 1000, Slovenia

5

Laboratory for Characterization and Processing of Polymers, Faculty of Mechanical Engineering, University of Maribor, Smetanova 17, Maribor 2000, Slovenia





*

Author to whom correspondence should be addressed.



Abstract Protein adhesion and cell response to plasma-treated polymer surfaces were studied. The polymer polyethylene terephthalate PET was treated in either an oxygen plasma to make the surface hydrophilic, or a tetrafluoromethane CF4 plasma to make the surface hydrophobic. The plasma source was radiofrequency RF discharge. The adsorption of albumin and other proteins from a cell-culture medium onto these surfaces was studied using a quartz crystal microbalance QCM, X-ray photoelectron spectroscopy XPS and atomic force microscopy AFM. The cellular response to plasma-treated surfaces was studied as well using an MTT assay and scanning electron microscopy SEM. The fastest adsorption rate was found on the hydrophilic oxygen plasma-treated sample, and the lowest was found on the pristine untreated sample. Additionally, the amount of adsorbed proteins was higher for the oxygen-plasma-treated surface, and the adsorbed layer was more viscoelastic. In addition, cell adhesion studies support this finding because the best cell adhesion was observed on oxygen-plasma-treated substrates. View Full-Text

Keywords: oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance QCM oxygen and fluorine plasma treatment; polymer surface modification; protein adsorption; cell adhesion; quartz crystal microbalance QCM





Author: Nina Recek 1, Morana Jaganjac 2, Metod Kolar 1, Lidija Milkovic 3, Miran Mozetič 4, Karin Stana-Kleinschek 5 and Alenka Vesel 4,*

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents