Assessment of Extraction Parameters on Antioxidant Capacity, Polyphenol Content, Epigallocatechin Gallate EGCG, Epicatechin Gallate ECG and Iriflophenone 3-C-β-Glucoside of Agarwood Aquilaria crassna Young LeavesReport as inadecuate




Assessment of Extraction Parameters on Antioxidant Capacity, Polyphenol Content, Epigallocatechin Gallate EGCG, Epicatechin Gallate ECG and Iriflophenone 3-C-β-Glucoside of Agarwood Aquilaria crassna Young Leaves - Download this document for free, or read online. Document in PDF available to download.

1

Department of Food Technology, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Malaysia

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Department of Food Science, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 Serdang, Malaysia

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Department of Food Science and Nutrition, Faculty of Applied Sciences, UCSI University, No. 1, Jalan Menara Gading, UCSI Heights, Cheras, 56000 Kuala Lumpur, Malaysia





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Abstract The effects of ethanol concentration 0%–100%, v-v, solid-to-solvent ratio 1:10–1:60, w-v and extraction time 30–180 min on the extraction of polyphenols from agarwood Aquilaria crassna were examined. Total phenolic content TPC, total flavonoid content TFC and total flavanol TF assays and HPLC-DAD were used for the determination and quantification of polyphenols, flavanol gallates epigallocatechin gallate—EGCG and epicatechin gallate—ECG and a benzophenone iriflophenone 3-C-β-glucoside from the crude polyphenol extract CPE of A. crassna. 2,2-Diphenyl-1-picrylhydrazyl DPPH radical scavenging activity was used to evaluate the antioxidant capacity of the CPE. Experimental results concluded that ethanol concentration and solid-to-solvent ratio had significant effects p < 0.05 on the yields of polyphenol and antioxidant capacity. Extraction time had an insignificant influence on the recovery of EGCG, ECG and iriflophenone 3-C-β-glucoside, as well as radical scavenging capacity from the CPE. The extraction parameters that exhibited maximum yields were 40% v-v ethanol, 1:60 w-v for 30 min where the TPC, TFC, TF, DPPH, EGCG, ECG and iriflophenone 3-C-β-glucoside levels achieved were 183.5 mg GAE-g DW, 249.0 mg QE-g DW, 4.9 mg CE-g DW, 93.7%, 29.1 mg EGCG-g DW, 44.3 mg ECG-g DW and 39.9 mg iriflophenone 3-C-β-glucoside-g DW respectively. The IC50 of the CPE was 24.6 mg-L. View Full-Text

Keywords: agarwood Aquilaria crassna leaves; epigallocatechin gallate EGCG; epicatechin gallate ECG; iriflophenone 3-C-β-glucoside; polyphenol; antioxidant capacity agarwood Aquilaria crassna leaves; epigallocatechin gallate EGCG; epicatechin gallate ECG; iriflophenone 3-C-β-glucoside; polyphenol; antioxidant capacity





Author: Pei Yin Tay 1,3, Chin Ping Tan 1,* , Faridah Abas 2, Hip Seng Yim 3 and Chun Wai Ho 3,*

Source: http://mdpi.com/



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