Bactericidal Effect of Extracts and Metabolites of Robinia pseudoacacia L. on Streptococcus mutans and Porphyromonas gingivalis Causing Dental Plaque and Periodontal Inflammatory DiseasesReport as inadecuate




Bactericidal Effect of Extracts and Metabolites of Robinia pseudoacacia L. on Streptococcus mutans and Porphyromonas gingivalis Causing Dental Plaque and Periodontal Inflammatory Diseases - Download this document for free, or read online. Document in PDF available to download.

1

School of Biotechnology, Yeungnam University, Gyeongsan, Gyeongbuk 712-749, Korea

2

Wildlife Genetic Resources Center, National Institute of Biological Resources, Incheon 404-708, Korea

3

Ensoltek Co., Ltd., Techno 10-ro 51, Yuseong-gu, Daejeon 305-510, Korea



These authors contributed equally to this work.





*

Authors to whom correspondence should be addressed.



Academic Editor: Peter J. Rutledge

Abstract The mouth cavity hosts many types of anaerobic bacteria, including Streptococcus mutans and Porphyromonas gingivalis, which cause periodontal inflammatory diseases and dental caries. The present study was conducted to evaluate the antibacterial potential of extracts of Robinia pseudoacacia and its different fractions, as well as some of its natural compounds against oral pathogens and a nonpathogenic reference bacteria, Escherichia coli. The antibacterial activity of the crude extract and the solvent fractions hexane, chloroform, ethyl acetate and butanol of R. pseudoacacia were evaluated against S. mutans, P. gingivalis and E. coli DH5α by standard micro-assay procedure using conventional sterile polystyrene microplates. The results showed that the crude extract was more active against P. gingivalis 100% growth inhibition than against S. mutans 73% growth inhibition at 1.8 mg-mL. The chloroform and hexane fractions were active against P. gingivalis, with 91 and 97% growth inhibition, respectively, at 0.2 mg-mL. None of seven natural compounds found in R. pseudoacacia exerted an antibacterial effect on P. gingivalis; however, fisetin and myricetin at 8 µg-mL inhibited the growth of S. mutans by 81% and 86%, respectively. The crude extract of R. pseudoacacia possesses bioactive compounds that could completely control the growth of P. gingivalis. The antibiotic activities of the hexane and chloroform fractions suggest that the active compounds are hydrophobic in nature. The results indicate the effectiveness of the plant in clinical applications for the treatment of dental plaque and periodontal inflammatory diseases and its potential use as disinfectant for various surgical and orthodontic appliances. View Full-Text

Keywords: fisetin; myricetin; Porphyromonas gingivalis; Robinia pseudoacacia; Streptococcus mutans fisetin; myricetin; Porphyromonas gingivalis; Robinia pseudoacacia; Streptococcus mutans





Author: Jayanta Kumar Patra 1,†, Eun Sil Kim 2,†, Kyounghee Oh 2, Hyeon-Jeong Kim 3, Radhika Dhakal 1, Yangseon Kim 3,* and Kwang-Hyun Baek 1,*

Source: http://mdpi.com/



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