Isolation, Identification and Cytotoxicity of a New Noroleanane-Type Triterpene Saponin from Salicornia bigelovii Torr.Report as inadecuate




Isolation, Identification and Cytotoxicity of a New Noroleanane-Type Triterpene Saponin from Salicornia bigelovii Torr. - Download this document for free, or read online. Document in PDF available to download.

1

College of Life Science, Nanjing Agricultural University, Nanjing 210095, China

2

Jiangsu Provincial Key Laboratory for Coastal Wetland Bioresources, Institute of Botany, Jiangsu Province and Chinese Academy of Sciences-Nanjing Botanical Garden Mem. Sun Yat-Sen, Nanjing 210014, China



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Isabel C. F. R. Ferreira

Abstract Salicornia bigelovii Torr. has been consumed not only as a popular kind of vegetable, but also as a medicinal plant to treat hypertension, cephalalgia, scurvy and cancer. The present study was designed to investigate its chemical components and cytotoxic activity. A new noroleanane-type triterpene saponin, bigelovii C 1, was separated and purified from Salicornia bigelovii Torr., along with four known triterpene saponins 2–5. The structure of bigelovii C was elucidated as 3-O-6-O-butyl ester-β-D-glucuropyranosyl-23-aldehyde-30-norolean-12, 20 29-dien-28-oic acid-28-O-β-D-glucopyranoside, according to various spectroscopic analysis and chemical characteristics. Besides Compounds 3 and 5, bigelovii C had potent cytotoxicity against three human cancer cell lines, MCF7 breast cancer, Lovo colon cancer and LN229 glioblastoma, especially MCF7. Bigelovii C inhibited the growth of MCF7 cells in dose- and time-dependent manners. Flow cytometry analysis revealed that the percentage of apoptotic cells significantly increased upon bigelovii C treatment. Rh123 staining assay indicated that bigelovii C reduced the mitochondrial membrane potential. The mechanism of cell death by bigelovii C may be attributed to the downregulation of Bcl-2 and upregulation of Bax, cleaved caspase-9, caspase-7 and PARP. These results suggested that bigelovii C may impart health benefits when consumed and should be regarded as a potential chemopreventative agent for cancer. View Full-Text

Keywords: Salicornia bigelovii Torr.; nortriterpenoid saponins; cytotoxicity; MCF-7 human breast cancer cells; apoptosis Salicornia bigelovii Torr.; nortriterpenoid saponins; cytotoxicity; MCF-7 human breast cancer cells; apoptosis





Author: Fuqin Guan 1,2,†, Qizhi Wang 2,†, Ming Wang 2, Yu Shan 2, Yu Chen 2, Min Yin 2, Youyi Zhao 2, Xu Feng 1,2,* , Fei Liu 2 and Jianhua Zhang 2

Source: http://mdpi.com/



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