Inhibition of Tanshinone IIA, Salvianolic Acid A and Salvianolic Acid B on Areca Nut Extract-Induced Oral Submucous Fibrosis in VitroReport as inadecuate




Inhibition of Tanshinone IIA, Salvianolic Acid A and Salvianolic Acid B on Areca Nut Extract-Induced Oral Submucous Fibrosis in Vitro - Download this document for free, or read online. Document in PDF available to download.

1

Department of Microbiology and Immunology, Shantou University Medical College, Shantou 515041, China

2

Department of Veterinary Medicine, University of Maryland, College Park, MD 20742, USA





*

Authors to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Salvia miltiorrhiza Bunge has been reported to possess excellent antifibrotic activity. In this study, we have investigated the effect and mechanism of tanshinone IIA Tan-IIA, salvianolic acid A Sal-A and salvianolic acid B Sal-B, the important active compounds of Salvia miltiorrhiza Bunge, on areca nut extract ANE-induced oral submucous fibrosis OSF in vitro. Through human procollagen gene promoter luciferase reporter plasmid assay, hydroxyproline assay, gelatin zymography assay, qRT-PCR, ELISA and Western blot assay, the influence of these three compounds on ANE-stimulated cell viability, collagen accumulation, procollagen gene transcription, MMP-2-9 activity, MMP-1-13 and TIMP-1-2 expression, cytokine secretion and the activation of PI3K-AKT, ERK-JNK-p38 MAPK and TGF-β-Smads pathways were detected. The results showed that Tan-IIA, Sal-A and Sal-B could significantly inhibit the ANE-stimulated abnormal viability and collagen accumulation of mice oral mucosal fibroblasts MOMFs, inhibit the transcription of procollagen gene COL1A1 and COL3A1, increase MMP-2-9 activity, decrease TIMP-1-2 expression and inhibit the transcription and release of CTGF, TGF-β1, IL-6 and TNF-α; Tan-IIA, Sal-A and Sal-B also inhibited the ANE-induced activation of AKT and ERK MAPK pathways in MOMFs and the activation of TGF-β-Smads pathway in HaCaT cells. In conclusion, Tan-IIA, Sal-A and Sal-B possess excellent antifibrotic activity in vitro and can possibly be used to promote the rehabilitation of OSF patients. View Full-Text

Keywords: tanshinone IIA; salvianolic acid A; salvianolic acid B; areca nut; oral submucous fibrosis tanshinone IIA; salvianolic acid A; salvianolic acid B; areca nut; oral submucous fibrosis





Author: Jian-Ping Dai 1,* , Dan-Xia Zhu 1, Jiang-Tao Sheng 1, Xiao-Xuan Chen 1, Wei-Zhong Li 2, Ge-Fei Wang 1, Kang-Sheng Li 1,* and Yun Su 1,*

Source: http://mdpi.com/



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