Effective Immobilization of Agrobacterium sp. IFO 13140 Cells in Loofa Sponge for Curdlan BiosynthesisReport as inadecuate




Effective Immobilization of Agrobacterium sp. IFO 13140 Cells in Loofa Sponge for Curdlan Biosynthesis - Download this document for free, or read online. Document in PDF available to download.

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Postgraduate Program in Food Science, State University of Maringá UEM, Av. Colombo, 5790, Maringá-PR 87020-900, Brazil

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Pharmacy Department, State University of Maringá UEM, Av. Colombo, 5790, Maringá-PR 87020-900, Brazil

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Food Engineering Department, Federal Technological University of Paraná UTFPR, Via Rosalina Maria dos Santos, 1233, Campo Mourão-PR 87301-899, Brazil





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Academic Editor: Maurizio Battino

Abstract Curdlan production by Agrobacterium sp. IFO13140 immobilized on loofa sponge, alginate and loofa sponge with alginate was investigated. There was no statistically-significant difference in curdlan production when the microorganism was immobilized in different matrices. The loofa sponge was chosen because of its practical application and economy and because it provides a high stability through its continued use. The best conditions for immobilization on loofa sponge were 50 mg of cell, 200 rpm and 72 h of incubation, which provided a curdlan production 1.50-times higher than that obtained by free cells. The higher volumetric productivity was achieved by immobilized cells 0.09 g-L-h at 150 rpm. The operating stability was evaluated, and until the fourth cycle, immobilized cells retained 87.40% of the production of the first cycle. The immobilized cells remained active after 300 days of storage at 4 °C. The results of this study demonstrate success in immobilizing cells for curdlan biosynthesis, making the process potentially suitable for industrial scale-up. Additional studies may show a possible contribution to the reduction of operating costs. View Full-Text

Keywords: curdlan; Agrobacterium sp.; immobilization; mixed-level design; loofa sponge curdlan; Agrobacterium sp.; immobilization; mixed-level design; loofa sponge





Author: Camila Ortiz Martinez 1, Suelen Pereira Ruiz 1, Marcela Tiemi Nogueira 2, Evandro Bona 3, Márcia Portilho 2 and Graciette Matioli 1,2,*

Source: http://mdpi.com/



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