Antibacterial Activity and Antibiotic-Enhancing Effects of Honeybee Venom against Methicillin-Resistant Staphylococcus aureusReport as inadecuate




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1

Rural Development Administration, National Academy of Agricultural Science, Wanju, Chonbuk 55365, Korea

2

School of Biomedical Sciences, Charles Sturt University, Bathurst 2795, Australia





*

Author to whom correspondence should be addressed.



Academic Editor: Mary Fletcher

Abstract Methicillin-resistant Staphylococcus aureus MRSA, along with other antibiotic resistant bacteria, has become a significant social and clinical problem. There is thus an urgent need to develop naturally bioactive compounds as alternatives to the few antibiotics that remain effective. Here we assessed the in vitro activities of bee venom BV, alone or in combination with ampicillin, penicillin, gentamicin or vancomycin, on growth of MRSA strains. The antimicrobial activity of BV against MRSA strains was investigated using minimum inhibitory concentrations MIC, minimum bactericidal concentrations MBC and a time-kill assay. Expression of atl which encodes murein hydrolase, a peptidoglycan-degrading enzyme involved in cell separation, was measured by reverse transcription-polymerase chain reaction. The MICs of BV were 0.085 µg-mL and 0.11 µg-mL against MRSA CCARM 3366 and MRSA CCARM 3708, respectively. The MBC of BV against MRSA 3366 was 0.106 µg-mL and that against MRSA 3708 was 0.14 µg-mL. The bactericidal activity of BV corresponded to a decrease of at least 3 log CFU-g cells. The combination of BV with ampicillin or penicillin yielded an inhibitory concentration index ranging from 0.631 to 1.002, indicating a partial and indifferent synergistic effect. Compared to ampicillin or penicillin, both MRSA strains were more susceptible to the combination of BV with gentamicin or vancomycin. The expression of atl gene was increased in MRSA 3366 treated with BV. These results suggest that BV exhibited antibacterial activity and antibiotic-enhancing effects against MRSA strains. The atl gene was increased in MRSA exposed to BV, suggesting that cell division was interrupted. BV warrants further investigation as a natural antimicrobial agent and synergist of antibiotic activity. View Full-Text

Keywords: bee venom; MRSA; antibacterial effect; antibiotic effects; atl bee venom; MRSA; antibacterial effect; antibiotic effects; atl





Author: Sang Mi Han 1,* , Joung Min Kim 1, In Pyo Hong 1, Soon Ok Woo 1, Se Gun Kim 1, He Rye Jang 1 and Sok Cheon Pak 2

Source: http://mdpi.com/



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