Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. ‘Liscia’ and C. medica cv. ‘Rugosa’ Cultivated in Southern ItalyReport as inadecuate


Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. ‘Liscia’ and C. medica cv. ‘Rugosa’ Cultivated in Southern Italy


Chemical Composition and in Vitro Antimicrobial, Cytotoxic, and Central Nervous System Activities of the Essential Oils of Citrus medica L. cv. ‘Liscia’ and C. medica cv. ‘Rugosa’ Cultivated in Southern Italy - Download this document for free, or read online. Document in PDF available to download.

1

Department of Pharmacy, University of Salerno, Via Giovanni Paolo II, 132, 84084 Fisciano Salerno, Italy

2

Istituto di Scienze dell’Alimentazione, Consiglio Nazionale delle Ricerche ISA-CNR, Via Roma 64, 83100 Avellino, Italy

3

Post-Doctoral by National Counsel of Technological and Scientific Development CNPq-Brazil, 70000-000 Brasília, Brazil





*

Author to whom correspondence should be addressed.



Academic Editor: Luca Forti

Abstract Citrus medica cv. ‘liscia’ and C. medica cv. ‘rugosa’ are two taxa of citron, belonging to the biodiversity of South Italy, in particular of Amalfi Coast, in the Campania region. The chemical composition of the essential oils EOs from fruit peels of both C. medica cultivars was studied by gas chromatography GC and gas chromatography-mass spectrometry GC-MS analyses. In all, 100 compounds were identified, 82 for C. medica cv. ‘liscia’, accounting for 91.4% of the total oil, and 88 for C. medica cv. ‘rugosa’, accounting for 92.0% of the total oil. Monoterpene hydrocarbons are the main constituents in both oils of C. medica cv. ‘liscia’ 79.1% and C. medica cv. ‘rugosa’ 80.2%. In both oils, limonene 67.2%–62.8% and camphene 8.5%–10.9% are the main constituents. The antimicrobial activity of the EOs was assayed against some bacterial strains: Bacillus cereus DSM 4313, Bacillus cereus DSM 4384, Staphylococcus aureus DSM 25693, Pseudomonas aeruginosa ATCC 50071, and Escherichia coli DSM 8579. Low concentrations of C. medica cv. ‘rugosa’ EO showed an inhibitory effect on P. aeruginosa and higher concentrations inhibited more B. cereus 4384 and E. coli than S. aureus. The cytotoxicity of the EO was evaluated against SH-SY5Y cell line. The influence of the EO on the expression of adenylate cyclase 1 ADCY1 was also studied. The antimicrobial activity registered confirm their traditional uses as food preserving agents and led us to hypothesize the possible use of these oils as antimicrobials. The alterations in ADCY1 expression suggested a role for limonene in effects on the central nervous system. View Full-Text

Keywords: Citrus medica; essential oil; antimicrobial activity; SH-SY5Y cells; adenylate cyclase Citrus medica; essential oil; antimicrobial activity; SH-SY5Y cells; adenylate cyclase





Author: Luigi Aliberti 1, Lucia Caputo 1, Vincenzo De Feo 1,* , Laura De Martino 1, Filomena Nazzaro 2 and Lucéia Fátima Souza 1,3

Source: http://mdpi.com/



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