Phytochemical Compositions of Immature Wheat Bran, and Its Antioxidant Capacity, Cell Growth Inhibition, and Apoptosis Induction through Tumor Suppressor GeneReport as inadecuate


Phytochemical Compositions of Immature Wheat Bran, and Its Antioxidant Capacity, Cell Growth Inhibition, and Apoptosis Induction through Tumor Suppressor Gene


Phytochemical Compositions of Immature Wheat Bran, and Its Antioxidant Capacity, Cell Growth Inhibition, and Apoptosis Induction through Tumor Suppressor Gene - Download this document for free, or read online. Document in PDF available to download.

1

Division of Funcitonal Food Research, Korea Food Research Institute, 62, Anyangpangyo-ro 1201 beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13539, Korea

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Division of Strategic Food Research, Korea Food Research Institute, 62, Anyangpangyo-ro 1201 beon-gil, Bundang-gu, Seongnam-si, Gyeonggi-do 13539, Korea





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Academic Editors: Isabel C. F. R. Ferreira and Nancy D. Turner

Abstract The purpose of this study was to investigate the phytochemical compositions and antioxidant capacity, cell growth inhibition, and apoptosis induction in extracts of immature wheat bran. Immature wheat bran IWB was obtained from immature wheat harvested 10 days earlier than mature wheat. The phytochemical compositions of bran extract samples were analyzed by ultra-high performance liquid chromatography. The total ferulic acid 3.09 mg-g and p-coumaric acid 75 µg-g in IWB were significantly higher than in mature wheat bran MWB, ferulic acid: 1.79 mg-g; p-coumaric acid: 55 µg-g. The oxygen radical absorbance capacity ORAC: 327 µM Trolox equivalents TE-g and cellular antioxidant activity CAA: 4.59 µM Quercetin equivalents QE-g of the IWB were higher than those of the MWB ORAC: 281 µM TE-g; CAA: 0.63 µM QE-g. When assessing cell proliferation, the IWB extracts resulted in the lowest EC50 values against HT-29 18.9 mg-mL, Caco-2 7.74 mg-mL, and HeLa cells 8.17 mg-mL among bran extract samples. Additionally, the IWB extracts increased the gene expression of p53 and PTEN tumor suppressor genes in HT-29 cells, indicating inhibited cell growth and induced apoptosis through tumor suppressor genes. View Full-Text

Keywords: immature wheat bran; phytochemical composition; antioxidant capacity; cell growth inhibition; tumor suppressor genes; apoptotic cell death immature wheat bran; phytochemical composition; antioxidant capacity; cell growth inhibition; tumor suppressor genes; apoptotic cell death





Author: Mi Jeong Kim 1, Won-Jin Yoon 2 and Sang Sook Kim 1,*

Source: http://mdpi.com/



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