A Flavone Constituent from Myoporum bontioides Induces M-Phase Cell Cycle Arrest of MCF-7 Breast Cancer CellsReport as inadecuate


A Flavone Constituent from Myoporum bontioides Induces M-Phase Cell Cycle Arrest of MCF-7 Breast Cancer Cells


A Flavone Constituent from Myoporum bontioides Induces M-Phase Cell Cycle Arrest of MCF-7 Breast Cancer Cells - Download this document for free, or read online. Document in PDF available to download.

1

Department of Marine Technology and Resources, National Sun-Yat-sen University, Kaohisung 804, Taiwan

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Division of Hematology and Oncology, Department of Internal Medicine, China Medical University Hospital, Taichung 404, Taiwan

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College of Medicine, China Medical University, Taichung 404, Taiwan

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Department of Pharmacy, Kinmen Hospital, Kinmen 891, Taiwan

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Cancer Center, China Medical University Hospital, Taichung 404, Taiwan

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School of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan

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School of Pharmacy, Taipei Medical University, Taipei 110, Taiwan

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Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan





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Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Abstract: Myoporum bontioides is a traditional medicinal plant in Asia with various biological activities, including anti-inflammatory and anti-bacterial characteristics. To identify the bioactive constituents from M. bontioides, a newly-identified flavone, 3,4′-dimethoxy-3′,5,7-trihydroxyflavone compound 1, along with eight known compounds, were investigated in human MCF-7 breast cancer, SCC4 oral cancer, and THP-1 monocytic leukemia cells. Among these compounds, compound 1 exhibited the strongest antiproliferative activity with half-maximal inhibitory concentration IC50 values ranging from 3.3 μM MCF-7 to 8.6 μM SCC4. Flow cytometric analysis indicated that compound 1 induced G2-M cell cycle arrest in MCF-7 cells. Mechanistic evidence suggests that the G2-M arrest could be attributable to compound 1’s modulatory effects on the phosphorylation and expression of numerous key signaling effectors, including cell division cycle 2 CDC2, CDC25C, and p53. Notably, compound 1 downregulated the expression of histone deacetylase 2 HDAC2 and HDAC4, leading to increased histone H3 acetylation and p21 upregulation. Together, these findings suggest the translational potential of compound 1 as a breast cancer treatment. View Full-Text

Keywords: Myoporum bontioides; Myoporaceae; flavone; cell cycle arrest; breast cancer Myoporum bontioides; Myoporaceae; flavone; cell cycle arrest; breast cancer





Author: Jing-Ru Weng 1,* , Li-Yuan Bai 2,3, Wei-Yu Lin 4, Chang-Fang Chiu 3,5, Yu-Chang Chen 6, Shi-Wei Chao 7 and Chia-Hsien Feng 8

Source: http://mdpi.com/



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