Quality Evaluation and Chemical Markers Screening of Salvia miltiorrhiza Bge. Danshen Based on HPLC Fingerprints and HPLC-MSn Coupled with ChemometricsReport as inadecuate


Quality Evaluation and Chemical Markers Screening of Salvia miltiorrhiza Bge. Danshen Based on HPLC Fingerprints and HPLC-MSn Coupled with Chemometrics


Quality Evaluation and Chemical Markers Screening of Salvia miltiorrhiza Bge. Danshen Based on HPLC Fingerprints and HPLC-MSn Coupled with Chemometrics - Download this document for free, or read online. Document in PDF available to download.

School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China





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Academic Editor: Derek J. McPhee

Abstract Danshen, the dried root of Salvia miltiorrhiza Bge., is a widely used commercially available herbal drug, and unstable quality of different samples is a current issue. This study focused on a comprehensive and systematic method combining fingerprints and chemical identification with chemometrics for discrimination and quality assessment of Danshen samples. Twenty-five samples were analyzed by HPLC-PAD and HPLC-MSn. Forty-nine components were identified and characteristic fragmentation regularities were summarized for further interpretation of bioactive components. Chemometric analysis was employed to differentiate samples and clarify the quality differences of Danshen including hierarchical cluster analysis, principal component analysis, and partial least squares discriminant analysis. Consistent results were that the samples were divided into three categories which reflected the difference in quality of Danshen samples. By analyzing the reasons for sample classification, it was revealed that the processing method had a more obvious impact on sample classification than the geographical origin, it induced the different content of bioactive compounds and finally lead to different qualities. Cryptotanshinone, trijuganone B, and 15,16-dihydrotanshinone I were screened out as markers to distinguish samples by different processing methods. The developed strategy could provide a reference for evaluation and discrimination of other traditional herbal medicines. View Full-Text

Keywords: Salvia miltiorrhiza; fingerprint; chemometric; HPLC-MSn; chemical marker; quality evaluation Salvia miltiorrhiza; fingerprint; chemometric; HPLC-MSn; chemical marker; quality evaluation





Author: Wenyi Liang, Wenjing Chen, Lingfang Wu, Shi Li, Qi Qi, Yaping Cui, Linjin Liang, Ting Ye and Lanzhen Zhang *

Source: http://mdpi.com/



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