Effects of Momordica charantia Bitter Melon on Ischemic Diabetic MyocardiumReport as inadecuate


Effects of Momordica charantia Bitter Melon on Ischemic Diabetic Myocardium


Effects of Momordica charantia Bitter Melon on Ischemic Diabetic Myocardium - Download this document for free, or read online. Document in PDF available to download.

Faculty of Pharmacy, Department of Pharmacology, University of Debrecen, Debrecen 4032, Hungary





*

Author to whom correspondence should be addressed.



Academic Editor: Derek J. McPhee

Abstract Objective: A rat model is here used to test a hypothesis that Momordica charantia Bitter melon BM extract favorably alters processes in cardiovascular tissue and is systemically relevant to the pathophysiology of type 2 diabetes T2DM and related cardiovascular disease. Methods: Male Lean and Zucker Obese ZO rats were gavage-treated for six weeks with 400 mg-kg body weight bitter melon BM extract suspended in mucin–water vehicle, or with vehicle Control. Animals were segregated into four treatment groups, 10 animals in each group, according to strain Lean or ZO and treatment Control or BM. Following six-week treatment periods, peripheral blood was collected from selected animals, followed by sacrifice, thoracotomy and mounting of isolated working heart setup. Results: Body mass of both Lean and ZO rats was unaffected by treatment, likewise, peripheral blood fasting glucose levels showed no significant treatment-related effects. However, some BM treatment-related improvement was noted in postischemic cardiac functions when Lean, BM-treated animals were compared to vehicle treated Lean control rats. Treatment of Lean, but not ZO, rats significantly reduced the magnitude of infarcted zone in isolated hearts subjected to 30 min of ischemia followed by 2 h of working mode reperfusion. Immunohistochemical demonstration of caspase-3 expression by isolated heart tissues subjected to 30 min of ischemia followed by 2 h of reperfusion, revealed significant correlation between BM treatment and reduced expression of this enzyme in hearts obtained from both Lean and ZO animals. The hierarchy and order of caspase-3 expression from highest to lowest was as follows: ZO rats receiving vehicle > ZO rats receiving BM extract > Lean rats treated receiving vehicle > Lean rats administered BM extract. Outcomes of analyses of peripheral blood content of cardiac-related analytics: with particular relevance to clinical application was a significant elevation in blood of ZO and ZO BM-treated, versus Lean rats of total cholesterol high density lipoprotein HDL-c + low density lipoprotein LDL-c, with an inferred increase in HDL-c-LDL-c ratio—an outcome associated with decreased risk of atherosclerotic disease. Conclusions: BM extract failed to positively affect T2DM- and cardiovascular-related outcomes at a level suggesting use as a standalone treatment. Nevertheless, the encouraging effects of BM in enhancement of cardiac function, suppression of post-ischemic-reperfused infarct size extent and capacity to modulate serum cholesterol, will likely make it useful as an adjuvant therapy for the management of T2DM and related cardiovascular diseases. View Full-Text

Keywords: ischemia; diabetes; bitter melon ischemia; diabetes; bitter melon





Author: Attila Czompa, Alexandra Gyongyosi, Kitti Szoke, Istvan Bak, Evelin Csepanyi, David D. Haines, Arpad Tosaki and Istvan Lekli *

Source: http://mdpi.com/



DOWNLOAD PDF




Related documents