Assessment of the Anti-Hyperglycaemic, Anti-Inflammatory and Antioxidant Activities of the Methanol Extract of Moringa Oleifera in Diabetes-Induced Nephrotoxic Male Wistar RatsReport as inadecuate


Assessment of the Anti-Hyperglycaemic, Anti-Inflammatory and Antioxidant Activities of the Methanol Extract of Moringa Oleifera in Diabetes-Induced Nephrotoxic Male Wistar Rats


Assessment of the Anti-Hyperglycaemic, Anti-Inflammatory and Antioxidant Activities of the Methanol Extract of Moringa Oleifera in Diabetes-Induced Nephrotoxic Male Wistar Rats - Download this document for free, or read online. Document in PDF available to download.

1

Phytomedicine and Diabetes Research Group, Oxidative Stress Research Centre, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa

2

Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, P.O. Box 1906, Bellville 7535, South Africa





*

Author to whom correspondence should be addressed.



Academic Editor: Rosa M. Lamuela-Raventós

Abstract Diabetes mellitus is an endocrine disease of multiple aetiologies in insulin secretion. A deficiency in insulin results in hyperglycemia with metabolic disturbances of biomolecules. Moringa oleifera MO is endemic in the tropics with a variety of ethnomedicinal importance. The leaf of this plant has been reported to possess antioxidant and medicinal properties that may be helpful in the treatment and management of diabetes and its associated complications. Diabetes was induced intraperitoneally in rats by a single dose of streptozotocin 55 mg-kg and treated with methanolic extract of Moringa oleifera 250 mg-kg b.wt for six weeks. Forty-eight 48 adult male Wistar strain rats were randomly divided into four groups: normal control NC, Moringa oleifera treated control rats NC + MO, diabetic rats DM and Moringa oleifera treated diabetic rats DM + MO. Estimation of antioxidant capacity, total polyphenols, flavonoids and flavonols content of Moringa oleifera extract was performed and serum biochemical markers were evaluated. Antioxidants such as catalase CAT, glutathione peroxidase GPx, superoxide dismutase SOD activities, glutathione GSH and inflammatory biomarkers were determined in the kidney. Results showed high antioxidant capacities of MO extract and improved serum biochemical markers, whilst lipid peroxidation MDA levels were reduced in non-diabetic and diabetic rats after MO treatment when compared to normal control. Subsequent administration of MO led to an increased concentration of serum albumin, globulin and total protein with a decrease in the level of MDA, and improvements in CAT, SOD, GSH, GPx, tumour necrosis factor-alphaTNF-α and interleukin-6IL-6. MO contains potent phytochemical constituents that offer protective action against diabetic-induced renal damage, reactive oxygen species ROS and inflammation and could therefore play a role in reducing diabetic complications, particularly in developing countries such as in Africa where the majority cannot afford orthodox medicine. View Full-Text

Keywords: Moringa oleifera; diabetes; methanolic extract; streptozotocin; hyperglycemia; nephrotoxicity; phytochemical; antioxidant; flavonoids; inflammatory Moringa oleifera; diabetes; methanolic extract; streptozotocin; hyperglycemia; nephrotoxicity; phytochemical; antioxidant; flavonoids; inflammatory





Author: Elizabeth I. Omodanisi 1, Yapo G. Aboua 2 and Oluwafemi O. Oguntibeju 1,*

Source: http://mdpi.com/



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