Effect of Temperature on Isolation and Characterization of Hydroxyapatite from Tuna Thunnus obesus BoneReport as inadecuate


Effect of Temperature on Isolation and Characterization of Hydroxyapatite from Tuna Thunnus obesus Bone


Effect of Temperature on Isolation and Characterization of Hydroxyapatite from Tuna Thunnus obesus Bone - Download this document for free, or read online. Document in PDF available to download.

1

Department of Chemistry, Pukyong National University, Busan 608-737, Korea

2

Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea





*

Author to whom correspondence should be addressed.



Abstract The effect of temperature on isolation and characterization of hydroxyapatite HAp from tuna bone was evaluated at different temperatures ranging from 200 °C to 1200 °C. The calcined bones were characterized by thermo gravimetric analysis TGA, Fourier transform infrared spectroscopy FTIR, X-ray diffraction XRD, field emission scanning electron microscopy FE-SEM, energy dispersive X-ray spectroscopy EDX and cytotoxicity assay. The FTIR and TGA results revealed the presence of inorganic and organic matrices in raw bone and a preserved carbonated group in the derived HAp. The XRD results of the derived HAp were coherent with the Joint Committee on Powder Diffraction Standards JCPDS-09-0432-1996 data. In addition, FE-SEM results revealed the formation of nanostructured HAp 80–300 nm at 600 °C and crystal agglomeration was observed with an increase in temperature. The calcium to phosphorous weight ratio was determined by EDX results of treated bones. Derived HAp with various crystal sizes had no cytotoxicity on the MG 63 cell line. Based on the analysis, we conclude that varying the isolation temperature between 600–900 °C has tremendous impact on the production of HAp from Thunnus obesus with required properties. View Full-Text

Keywords: Thunnus obesus; thermal calcinations; hydroxyapatite; X-ray diffraction; cytotoxicity Thunnus obesus; thermal calcinations; hydroxyapatite; X-ray diffraction; cytotoxicity





Author: Jayachandran Venkatesan 1 and Se Kwon Kim 1,2,*

Source: http://mdpi.com/



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