Influence of Amphibian Antimicrobial Peptides and Short Lipopeptides on Bacterial Biofilms Formed on Contact LensesReport as inadecuate




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1

Department of Inorganic Chemistry, Faculty of Pharmacy, Medical University of Gdansk, Gdansk 80-416, Poland

2

Pharmaceutical Laboratory, Avena SJ, Osielsko 86-031, Poland



These authors contributed equally to this work.





*

Author to whom correspondence should be addressed.



Academic Editor: Mauro Pollini

Abstract The widespread use of contact lenses is associated with several complications, including ocular biofilm-related infections. They are very difficult to manage with standard antimicrobial therapies, because bacterial growth in a biofilm is associated with an increased antibiotic resistance. The principal aim of this study was to evaluate the efficacy of antimicrobial peptides AMPs in eradication of bacterial biofilms formed on commercially available contact lenses. AMPs were synthesized according to Fmoc-tBu chemistry using the solid-phase method. Minimum inhibitory concentration MIC and minimum biofilm eradication concentration MBEC of the compounds were determined. Anti-biofilm activity of the antimicrobial peptides determined at different temperatures 25 °C and 37 °C were compared with the effectiveness of commercially available contact lens solutions. All of the tested compounds exhibited stronger anti-biofilm properties as compared to those of the tested lens solutions. The strongest activity of AMPs was noticed against Gram-positive strains at a temperature of 25 °C. Conclusions: The results of our experiments encourage us toward further studies on AMPs and their potential application in the prophylaxis of contact lens-related eye infections. View Full-Text

Keywords: antimicrobial peptides; lipopeptides; biofilm; ocular infections; contact lenses antimicrobial peptides; lipopeptides; biofilm; ocular infections; contact lenses





Author: Magdalena Maciejewska 1,2,†, Marta Bauer 1,†, Damian Neubauer 1, Wojciech Kamysz 1 and Malgorzata Dawgul 1,*

Source: http://mdpi.com/



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